edited by Thomas Kroneis, Institute of Cell Biology, Histology & Embryology, Medical University of Graz, Graz, Austria.
.PUBLICATION, DISTRIBUTION, ETC
Place of Publication, Distribution, etc.
New York :
Name of Publisher, Distributor, etc.
Humana Press,
Date of Publication, Distribution, etc.
[2015]
SERIES
Series Title
Methods in molecular biology,
Series Title
Springer protocols,
Volume Designation
v. 1347
ISSN of Series
1064-3745 ;
ISSN of Series
1949-2448
INTERNAL BIBLIOGRAPHIES/INDEXES NOTE
Text of Note
Includes bibliographical references and index.
SUMMARY OR ABSTRACT
Text of Note
This volume mirrors the holistic feature of whole genome amplification (WGA) technology by combining reviews, detailed basic methods and advanced sample workflows. The first part of the book covers an overview of the development of WGA techniques throughout recent years including general considerations on bias in WGA, possible sample pre-enrichment strategies and how to run a single-cell lab. The second part focuses on major WGA methods and protocols that allow the assessment of WGA product quality. The final chapters contain advanced protocols and address issues such as sample preparation using laser-micro dissection; WGA from partially degraded DNA (formalin-fixed paraffin embedded samples); circulating tumor cells; and ancient samples.--