عنوان

Biotechnology of plant secondary metabolism

(Number (ISBN

9781493933914 )alk. paper(

Title Proper

Biotechnology of plant secondary metabolism

First Statement of Responsibility

edited by Arthur Germano Fett-Neto, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil

Name of Publisher, Distributor, etc.

Springer

Date of Publication, Distribution, etc.

2016

Specific Material Designation and Extent of Item

xi, 180 pages : illustrations )some color(

Series Title

Methods in molecular biology,1064-3745 ;1405

Series Title

Springer protocols

Series Title

Series: Methods in molecular biology )Clifton, N.J.( ;v. 1405.1064-3745

Series Title

Series: Springer protocols )Series(1949-2448

Text of Note

This volume describes up-to-date techniques for improved production of secondary metabolites of economic interest using field and laboratory methods. Biotechnology of Plant Secondary Metabolism: Methods and Protocols explores different secondary metabolite classes, whole-plant and cell/organ culture systems, and environmental and genetic transformation-based modulation of biochemical pathways. Special focus is given to cell and tissue specific metabolism, metabolite transport, microRNA-based technology, heterologous systems expression of enzymes and pathways leading to products of interest, as well as applications using both model and non-model plant species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and cutting-edge, Biotechnology of Plant Secondary Metabolism: Methods and Protocols is a great resource for scientists of interdisciplinary fields--plant science, plant physiology, pharmacy, molecular biology, biochemistry, bioengineering, and forestry--in reaching their goals of producing plant biochemicals in a sustainable and efficient manner, while minimizing impacts to the environment and providing the required quantities of these commodities to industry.--Source other than Library of Congress.

Text of Note

Elicitation approaches for withanolide production in hairy root culture of Withania somnifera )L.( Dunal /Ganeshan Sivanandhan ]and others[ --Stimulant paste preparation and bark streak tapping technique for pine oleoresin extraction /Thanise Nogueira Feuller ]and others[ --Modified protocol for high-quality RNA extraction from oleoresin-producing adult pines /Jaulio Caesar de Lima ]and others[ --Collection of apoplastic fluids from Arabidopsis thaliana leaves /Svend Roesen Madsen, Hussam Hassan Nour-Eldin, and Barbara Ann Halkier --From plant extract to a cDNA encoding a glucosyltransferase candidate : proteomics and transcriptomics as tools to help elucidate saponin biosynthesis in Centella asiatica /Fernanda de Costa ]and others[ --Production of recombinant caffeine synthase from guarana )Paullinia cupana var. sorbilis( in Escherichia coli /Flaavia Camila Schimpl, Eduardo Kiyota, and Paulo Mazzafera --Heterologous expression and characterization of mimosinase from Leucaena leucocephala /Vishal Singh Negi and Dulal Borthakur --Production of aromatic plant terpenoids in recombinant baker's yeast /Anita Emmerstorfer-Augustin and Harald Pichler --Purification of a recombinant polyhistidine-tagged glucosyltransferase using immobilized metal-affinity chromatography )IMAC( /Fernanda de Costa ]and others[ --Western blot protocol for detection of proteins heterologously expressed in Xenopus laevis oocytes /Morten Egevang J▓rgensen, Hussam Hassan Nour-Eldin, and Barbara Ann Halkier --Laser capture microdissection : avoiding bias in analysis by selecting just what matters /Maarcia R. de Almeida and Martina V. Streomvik --Analytical and fluorimetric methods for the characterization of the transmembrane transport of specialized metabolites in plants /Inaes Carqueijeiro ]and others[ --Protoplast transformation as a plant-transferable transient expression system /Patraicia Duarte ]and others[ --Design, construction, and validation of artificial MicroRNA vectors using agrobacterium-mediated transient expression system /Basdeo Bhagwat ]and others[ --Knockdown of polyphenol oxidase gene expression in potato )Solanum tuberosum L.( with artificial MicroRNAs /Ming Chi ]and others[.

Text of Note

Includes bibliographical references and index.

Variant Title

methods and protocols

Entry Element

Plant cells and tissues^aLaboratory manuals

Entry Element

Plants^ametabolism

Entry Element

Plants^agenetics

Entry Element

Secondary Metabolism

Entry Element

Biotechnology

Entry Element

Laboratory Manuals

Entry Element

Plant cells and tissues.^afast--)OCoLC(fst01065318

Entry Element

Laboratory manuals.^afast--)OCoLC(fst01920776

Class number

Classification Record Number

Book number

Fett-Neto, Arthur Germano

1967

English Book