عنوان

miRNA maturation

(Number (ISBN

9781627037020 )alk. paper(

Title Proper

miRNA maturation

First Statement of Responsibility

edited by Christoph Arenz, Institute for Chemistry, Humboldt-Universiteat zu Berlin, Berlin, Germany

Specific Material Designation and Extent of Item

x, 192 pages : illustrations )some color( ;

Series Title

Methods in molecular biology,1064-3745 ;1095

Series Title

Springer protocols

Series Title

Series: Methods in molecular biology )Clifton, N.J.( ;v. 1095.1064-3745

Series Title

Series: Springer protocols )Series(1949-2448

Text of Note

Pathway of miRNA maturation /Michael Sand --MicroRNA maturation and human disease /Marlen Hesse and Christoph Arenz --Approaches to the modulation of miRNA maturation /Valerie T. Tripp, Jaclyn R. McKenna, and Douglas D. Young --Expression profiling of components of the miRNA maturation machinery /Michael Sand and Marina Skrygan --Primary MicroRNA processing assay reconstituted using recombinant Drosha and DGCR8 /Ian Barr and Feng Guo --In vivo processing assay based on a dual-luciferase reporter system to evaluate DROSHA enzymatic activity /Vera Bilan ... ]et al.[ --Assaying dicer-mediated miRNA maturation by means of fluorescent Substrates /Marlen Hesse, Brian P. Davies, and Christoph Arenz --Fluorescence correlation spectroscopy-based enzyme assay for human dicer /Eileen Magbanua and Ulrich Hahn --Detection of microRNA maturation using unmodified pre-microRNA and branched rolling circle amplification /Saskia Neubacher and Christoph Arenz --Quantitative RT-PCR specific for precursor and mature miRNAs /Hannah Zeollner, Stephan A. Hahn, and Abdelouahid Maghnouj --Cellular microRNA sensors based on luciferase reporters /Colleen M. Connelly and Alexander Deiters --Identification of inhibitors of microRNA function from small molecule screens /Colleen M. Connelly and Alexander Deiters --Inhibition of miRNA maturation by peptide nucleic acids /Concetta Avitabile ... ]et al.[ --Molecular methods for validation of the biological activity of peptide nucleic acids targeting microRNAs /Eleonora Brognara ... ]et al.[ --Lentiviral overexpression of miRNAs /Hannah Zeollner, Stephan A. Hahn, and Abdelouahid Maghnouj.

Text of Note

In miRNA Maturation: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study miRNA maturation. These included established methods such as fluorescent and non-fluorescent methods for homogenous assays of Dicer-mediated miRNA maturation or an in vivo assay for Drosha activity. Moreover, the volume also contains useful, but less-common methods that are hard to find elsewhere. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, miRNA Maturation: Methods and Protocols seeks to widen the view on miRNA as biological mediator and potential drug target.

Text of Note

Includes bibliographical references and index.

Variant Title

methods and protocols

Entry Element

RNA^aLaboratory manuals

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MicroRNAs^ametabolism--Laboratory Manuals

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Laboratory Manuals.^amesh

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RNA.^afast--)OCoLC(fst01086244

Entry Element

Handbooks, manuals, etc.^afast--)OCoLC(fst01423877

Number

Class number

Classification Record Number

Book number

Arenz, Christoph

Title: Micro RNA maturation

English Book