عنوان

Gene regulation

(Number (ISBN

9781627032834 )alk. paper(

Title Proper

Gene regulation

First Statement of Responsibility

edited by Minou Bina

Place of Publication, Distribution, etc.

New York

Name of Publisher, Distributor, etc.

Humana Press ;Springer,

Date of Publication, Distribution, etc.

c2013

Specific Material Designation and Extent of Item

xv, 401 p. : ill. )some col.(

Series Title

Methods in molecular biology ;v. 977

Series Title

Series: Methods in molecular biology )Clifton, N.J.( ;v. 977.

Text of Note

Gene regulation /Minou Bina --Isolation of nuclei for use in genome-wide DNase hypersensitivity assays to probe chromatin structure /Guoyu Ling and David J. Waxman --DNase I digestion of isolated nulcei for genome-wide mapping of DNase hypersensitivity sites in chromatin /Guoyu Ling and David J. Waxman --Isolation and analysis of DNA derived from nucleosome-free regions /Matthew Murtha ... ]et al.[ --Acquisition of high quality DNA for massive parallel sequencing by in vivo chromatin immunoprecipitation /M. van den Boogaard ... ]et al.[ --Luciferase assay to study the activity of a cloned promoter DNA fragment /Nina Solberg and Stefan Krauss --Promoter deletion analysis using a dual-luciferase reporter system /Yong Zhong Xu ... ]et al.[ --Application of mRNA display for in vitro selection of DNA-binding transcription factor complexes /Seiji Tateyama and Hiroshi Yanagawa --Isolation of intracellular protein - DNA complexes using HaloCHIP an antibody-free alternative to chromatin immunoprecipitation /Danette L. Daniels and Marjeta Urh --Modified yeast one-hybrid system for genome-wide identification of transcription factor binding sites /Kazuyuki Yanai --Identifying specific protein-DNA interactions using SILAC-based quantitative proteomics /Cornelia G. Spruijt, H. Irem Baymaz, and Michiel Vermeulen --Electrophoretic mobility-shift and super-shift assays for studies and characterization of protein-DNA complexes /Elsie I. Pares-Matos --Combination of native and denaturing PAGE for the detection of protein binding regions in long fragments of genomic DNA /Kristel Kaer and Mart Speek --Quantitative nanoproteomics approach for protein complex )QNanoPX( using gold nanoparticle-based DNA probe /Shu-Hui Chen and Mei-Yin Lin --Chromatin assembly and in vitro transcription analyses for evaluation of individual protein activities in multicomponent transcriptional complexes /Takayuki Furumatsu and Hiroshi Asahara --Using FRET to monitor protein-induced DNA bending : the TBP-TATA complex as a model system /Rebecca H. Blair, James A. Goodrich, and Jennifer F. Kugel --Promoter independent abortive transcription assays unravel functional interactions between TFIIB and RNA polymerase /Simone C. Wiesler, Finn Werner, and Robert O.J. Weinzierl --Fluorescence cross-correlation spectroscopy )FCCS( to observe dimerization of transcription factors in living cells /Hisayo Sadamoto and Hideki Muto ---Nuclear recruitment assay as a tool to validate transcription factor interactions in mammalian cells /C.J.J. Boogerd, V.M. Christoffels, and P. Barnett --Preparation of cell lines for single-cell analysis of transcriptional activation dynamics /Ilona U. Rafalska-Metcalf and Susan M. Janicki --Peptide microarrays for profiling of serine/threonine kinase activity of recombinant kinases and lysates of cells and tissue samples /Riet Hilhorst ... ]et al.[ --Immunoaffinity purification of protein complexes from mammalian cells /Chieri Tomomori-Sato ... ]et al.[ --Simple and efficient identification of chromatin modifying complexes and characterization of complex composition /Jeong-Heon Lee and David Skalnik --Heavy methyl-SILAC labeling coupled with liquid chromatography and high-resolution mass spectrometry to study the dynamics of site-specific histone methylation /Xing-Jun Cao, Barry M. Zee, and Benjamin A. Garcia --Analysis of p300 occupancy at the early stage of stem cell differentiation by chromatin immunoprecipitation /Melanie Le May and Qiao Li --Mammalian two-hybrid assays for studies of interaction of p300 with transcription factors /Daniela B. Mendonca, Gustavo Mendonca, and Lyndon F. Cooper --Fluorescence anisotropy microplate assay to investigate the interaction of full-length steroid receptor coactivator-1a with steroid receptors /Chen Zhang, Steven K. Nordeen, and David J. Shapiro --Use of histone deacetylase inhibitors to examine the roles of bromodomain and histone acetylation in p300-dependent gene expression /Jihong Chen and Qiao Li --Histone deacetylase inhibitor valproic acid as a small molecule inducer to direct the differentiation of pluripotent stem cells /Jihong Chen, Natascha Lacroix, and Qiao Li --Sedimentation and immunoprecipitation assays for analyzing complexes that repress transcription /Ping Lu ... ]et al.[ --Methods for studies of protein interactions with different DNA methyltransferases /Jianchang Yang.

Text of Note

Includes bibliographical references and index.

Variant Title

methods and protocols

Entry Element

Genetic regulation^aLaboratory manuals

Class number

Classification Record Number

Book number

Bina, Minou

English Book