عنوان

Gene essentiality

(Number (ISBN

9781493923977 )alk. paper(

Title Proper

Gene essentiality

First Statement of Responsibility

edited by Long Jason Lu, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA

Specific Material Designation and Extent of Item

xi, 248 pages : illustrations )some color(

Series Title

Methods in molecular biology,1064-3745 ;1279

Series Title

Springer protocols

Series Title

Series: Methods in molecular biology )Clifton, N.J.( ;v. 1279.1064-3745

Series Title

Series: Springer protocols )Series(1949-2448

Text of Note

Microarray Transposon Tracking for the Mapping of Conditionally Essential Genes in Campylobacter jejuni --Identifying Essential Streptococcus sanguinis Genes Using Genome-Wide Deletion Mutation --Defining Essential Genes and Identifying Virulence Factors of Porphyromonas gingivalis by Massively-Parallel Sequencing of Transposon Libraries)Tn-seq( --Identification of Essential Genes and Synthetic Lethal Gene Combinations in Escherichia coli K-12 --Identification of Genes Essential for Leptospirosis --Identifying Essential Genes in Mycobacterium tuberculosis by Global Phenotypic Profiling --Essential Genes in the Infection Model of Pseudomonas aeruginosa-PCR-Based Signature-Tagged Mutagenesis --Genome-Wide Synthetic Genetic Screening by Transposon Mutagenesis in Candida albicans --An Integrated Machine-Learning Model to Predict Prokaryotic Essential Genes --A Statistical Framework for Improving Genomic Annotations of Transposon Mutagenesis)TM( Assigned Essential Genes --A Proposed Essential Gene Discovery Pipeline: A Campylobacter jejuni Case Study --Computational Prediction of Essential Metabolic Genes Using Constraint-Based Approaches --Three Computational Tools for Predicting Bacterial Essential Genes --Gene Essentiality Analysis Based on DEG 10, an Updated Database of Essential Genes --Discovering Essential Domains in Essential Genes.

Text of Note

"This volume opens by covering two main types of approaches widely used to determine essential genes: single-gene knockouts and transposon mutagenesis, in both prokaryotes and "Candida albicans." Given the significant advancement in the computational predictions of microbial essential genes, the second half of the book examines four main types of approaches: comparative genomics, supervised machine learning, constraint-based methods, and corrections of transposon mutagenesis data, as well as databases and servers that are often used in studying gene essentiality. Written in the highly successful "Methods in Molecular Biology" series format, chapters include an introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and up-to-date, "Gene Essentiality: Methods and Protocols" will aid researchers who wish to further our knowledge in this vital field of study."--Back cover.

Text of Note

Includes bibliographical references and index.

Variant Title

methods and protocols

Entry Element

Microbial genomics

Entry Element

Genomics

Entry Element

Genes, Essential^aLaboratory Manuals

Entry Element

Genetic Techniques^aLaboratory Manuals

Entry Element

Laboratory Manuals.^amesh

Entry Element

Genomics.^afast--)OCoLC(fst00940228

Entry Element

Microbial genomics.^afast--)OCoLC(fst01019505

Class number

Classification Record Number

Book number

Lu, Long Jason

English Book