عنوان

Enzyme engineering

(Number (ISBN

9781627032926 )alk. paper(

Title Proper

Enzyme engineering

First Statement of Responsibility

edited by James C. Samuelson

Place of Publication, Distribution, etc.

New York

Name of Publisher, Distributor, etc.

Humana Press ;Springer,

Date of Publication, Distribution, etc.

c2013

Specific Material Designation and Extent of Item

xi, 252 p. : ill. )some col.(

Series Title

Methods in molecular biology,1064-3745 ;978

Series Title

Springer protocols

Series Title

Series: Methods in molecular biology )Clifton, N.J.( ;v. 978.

Series Title

Series: Springer protocols )Series(

Text of Note

Tripartite fusion system for the selection of protein variants with increased stability in vivo /Linda Foit and James C.A. Bardwell --Determining enzyme kinetics via isothermal titration calorimetry /Neil A. Demarse ... ]et al.[ --GFP reporter screens for the engineering of amino acid degrading enzymes from libraries expressed in bacteria /Olga Paley ... ]et al.[ --Flow cytometric assays for interrogating LAGLIDADG homing endonuclease DNA-binding and cleavage properties /Sarah K. Baxter ... ]et al.[ --TAL effector nuclease )TALEN( engineering /Ting Li and Bing Yang --In vitro evolution of enzymes /Misha V. Golynskiy ... ]et al.[ --Residue-specific incorporation of unnatural amino acids into proteins in vitro and in vivo /Amrita Singh-Blom, Randall A. Hughes, and Andrew D. Ellington --Reconstructing evolutionary adaptive paths for protein engineering /Megan F. Cole ... ]et al.[ --Oligonucleotide recombination enabled site-specific mutagenesis in bacteria /Bryan M. Swingle --FX cloning : a versatile high-throughput cloning system for characterization of enzyme variants /Eric R. Geertsma --Use of Sulfolobus solfataricus PCNA subunit proteins to direct the assembly of multimeric enzyme complexes /Hidehiko Hirakawa and Teruyuki Nagamune --Gene synthesis by assembly of deoxyuridine-containing oligonucleotides /Romualdas Vaisvila and Jurate Bitinaite --Protein engineering : single or multiple site-directed mutagenesis /Pei-Chung Hsieh and Romualdas Vaisvila --Gene assembly and combinatorial libraries in S. cerevisiae via reiterative recombination /Nili Ostrov, Laura M. Wingler, and Virginia W. Cornish --Promiscuity-based enzyme selection for rational directed evolution experiments /Sandeep Chakraborty ... ]et al.[ --Rational protein sequence diversification by multi-codon scanning mutagenesis /Jia Liu and T. Ashton Cropp --Screening libraries for improved solubility : using E. coli dihydrofolate reductase as a reporter /Jian-Wei Liu and David L. Ollis --In vitro directed evolution of enzymes expressed by E. coli in microtiter plates /Bradley J. Stevenson, Sylvia H.-C. Yip, and David L. Ollis.

Text of Note

Includes bibliographical references and index.

Variant Title

methods and protocols

Entry Element

Enzymes^aBiotechnology

Entry Element

Enzymes^aIndustrial applications

Entry Element

Protein engineering

Entry Element

Enzymes^aLaboratory Manuals

Entry Element

Protein Engineering^amethods--Laboratory Manuals

Entry Element

Biomedical Engineering^amethods--Laboratory Manuals

Number

Class number

Classification Record Number

Book number

Samuelson, James C.

1968

English Book