DNA sequencing III : dealing with difficult templates
.PUBLICATION, DISTRIBUTION, ETC
Place of Publication, Distribution, etc.
Sudbury, Mass.
Name of Publisher, Distributor, etc.
Jones and Bartlett Publishers
Date of Publication, Distribution, etc.
c2009
PHYSICAL DESCRIPTION
Specific Material Designation and Extent of Item
xvi, 184 p., 8 p. of plates : ill. )some col.(
GENERAL NOTES
Text of Note
Includes bibliographical references and index
NOTES PERTAINING TO TITLE AND STATEMENT OF RESPONSIBILITY
Text of Note
edited by Jan Kieleczawa
CONTENTS NOTE
Text of Note
Controlled heat-denaturation of DNA plasmids and PCR fragments -- Heat denaturation is an effective step in sequencing of many difficult DNA templates -- Solutions for sequencing difficult regions -- Improving sequence results from difficult templates with Phi 92 DNA polymerase and nucleotide analogs: the TempliPhi sequence resolver kit -- Sequencing through various secondary structures: detailed studies of pDEST vectors and other DNA templates with hairpins -- Transcriptional sequencing as a tool for reading difficult-to-read templates -- Bias-free cloning of "unclonable" DNA for simplified genomic finishing -- Bioinformatics tools to aide sequencing of difficult templates