Hairpin RNAi in plants / Louisa Matthew -- Virus induced gene silencing (VIGS) to study gene function in plants / Xiaohong Zhu and Savithramma P. Dinesh-Kumar -- The use of artificial microRNAs in targeted gene silencing / John Paul Alvarez and Yuval Eshed -- RNAi in Caenorhabditis elegans / Nicholas M. Johnson, Julie-Anne Fritz, and Carolyn A. Behm -- RNAi in Drosophila / Steven Whyard -- RNAi in fish and crustaceans / Mario Pablo Estrada, Juana Maria Lugo, and Yamila Carpio -- RNAi in chicken embryos / Nick J. Van Hateren, Raman M. Das, and Stuart A. Wilson -- RNAi in mammalian cells / Yan Zeng -- SiRNA and shRNA design / Debra J. Taxman.