Overview of plant RNAi /Yuichiro Watanabe --Caveat of RNAi in plants : the off-target effect /Muthappa Senthil-Kumar and Kirankumar S. Mysore --Plant gateway vectors for RNAi as a tool for functional genomic studies /Toshiya Muranaka --Heat-inducible RNAi for gene functional analysis in plants /Frederic Masclaux and Jean-Philippe Galaud --Gene function analysis by artificial microRNAs in Physcomitrella patens /Basel Khraiwesh ... [et al.] -- Virus-induced gene silencing in ornamental plants /Cai-Zhong Jiang, Jen-Chih Chen, and Michael Reid --Local RNA silencing mediated by agroinfiltration /Jutta Maria Helm, Elena Dadami, and Kriton Kalantidis --Direct transfer of synthetic double-stranded RNA into protoplasts of Arabidopsis thaliana /Ha-il Jung, Zhiyang Zhai, and Olena K. Vatamaniuk --Detection of long and short double-stranded RNAs /Toshiyuki Fukuhara ... [ et al.] --Quantitative stem-loop RT-PCR for detection of microRNAs /Erika Varkonyi-Gasic and Roger P. Hellens --Large-scale sequencing of plant small RNAs /William P. Donovan, Yuanji Zhang, and Miya D. Howell --Computational prediction of plant miRNA targets /Ying-Hsuan Sun ... [et al.] --Bisulfite sequencing for cytosine-methylation analysis in plants /Nazmul Haque and Masamichi Nishiguchi --Using nuclear run-on transcription assays in RNAi studies /Basel Khraiwesh --Proteomic analysis of RNA interference induced knockdown plant /Sang Yeol Lee and Kyun Oh Lee --Comparative analysis of phosphoprotein expression using 2D-