عنوان

Investigating histological and stereological changes along with oxidative stress parameters and alternations of BDNF and TrkB genes expression in the prefrontal cortex of male rats during schizophrenia induction and treatment with quercetin liposomes

شماره

T28624

زبان متن نوشتاري يا گفتاري و مانند آن

انگلیسی

عنوان اصلي

Investigating histological and stereological changes along with oxidative stress parameters and alternations of BDNF and TrkB genes expression in the prefrontal cortex of male rats during schizophrenia induction and treatment with quercetin liposomes

نام عام مواد

Dissertation

نام نخستين پديدآور

Afrah Barbar Obaid,

نام ناشر، پخش کننده و غيره

Natural Sciences

تاریخ نشرو بخش و غیره

1401

نام خاص و کميت اثر

173p.

ساير جزييات

cd.

جزئيات پايان نامه و نوع درجه آن

M.S.

نظم درجات

Animal Physiology

زمان اعطا مدرک

1401/12/22

متن يادداشت

Schizophrenia is one of the heterogeneous psychiatric disorders that currently has no definitive treatment. Schizophrenia is a severe mental illness with a prevalence of approximately 0.4% worldwide. This disease is a heterogeneous disorder with positive symptoms (delusions, hallucinations, mental disorders), negative symptoms (anhedonia, avoidance, social withdrawal, poor thinking) and cognitive dysfunction. The underlying pathology of schizophrenia is directly related to oxidative stress. Oxidative stress is defined as a disruption of the prooxidant/antioxidant balance in favor of the former, leading to potential damage. Therefore, the reduction of antioxidants and/or the increase of RS production leads to oxidative damage of cellular lipids, proteins, enzymes, carbohydrates and DNA. This disease also causes anxiety-like behaviors. Antioxidants are commonly used to counteract the oxidative stress effects. In this research, we used Quercetin and liposome quercetin as an antioxidant. BDNF and TrkB, are neurotrophins and involved in several pathways. Anxiety-like behaviors were also examined by Elevated Plus maze. Oxidative stress parameters such as malondialdehyde, superoxide dismutase, etc. were investigated. Histological and stereological changes were also investigated.Background and aim: 36 male Wistar rats with weight range of (250 ± 50 gr), were divided Randomly in 7 groups (n=8): including control (normal saline), ketamin (20 mg/kg), ethanol, quercetin (30 mg/kg), ketamin (20 mg/kg) + quercetin (30 mg/kg), ketamin (20 mg/kg) + liposome quercetin (30 mg/kg), liposome quercetin (30 mg/kg). In all group intraperitoneal injection were used. Injections were performed for 14 consecutive days. But in the groups receiving liposome, the duration of injection was 7 consecutive days. The study of the anxiety-like behavior of animals during the presentation of ketamin and quercetin were performed by using Elevated Plus Maze (EPM). At the end of the experiments, the animal's brain was removed from the skull and the Prefrontal cortex of the brain was transferred to a -80 degree freezer after incision for gene expression experiments. BDNF and TrkB expression levels were investigated by using the Real-time PCR technique. A part of brain tissue was transferred into formalin 15% for histological and stereological examination. Sections from the prefrontal cortex after freezing in liquid nitrogen until the assessment of stress indices oxidative were stored in the freezer at -80. The rest of the brain tissue was fixed in 10% buffered formalin for histological and stereological studies. In order to analyze the data, we used One-Way ANOVA and Instat3 software.Results: Results showed that there is no significant difference between groups in SOD level (P>0.05). The effect of intra -peritoneal injection of ketamine (20 mg /kg), quercetin antioxidant (30 mg /kg) and quercetin liposome (30 mg /kg) over the day 14 on the MalonDialdehyde parameter shows that There is a significant difference between the groups receiving ketamine (20 mg/kg) and quercetin (30 mg/kg) with the control group (P<0.05).There is no significant difference between other groups (P>0.05). The effect of intra -peritoneal injection of ketamine (20 mg /kg), quercetin antioxidant (30 mg /kg) and quercetin liposome (30 mg /kg) during the day 14 on the surface of the glutathione peroxidase indicates that there is no significant difference between groups (P>0.05). TAC level: The effect of intravenity of ketamine (20 mg /kg), quercetin antioxidant (30 mg /kg) and quercetin liposome (30 mg /kg) during the day 14 indicates that between the ketamine recipient group (20 mg /kg )+ Quercetin Liposome (30 mg /kg) there is a significant difference with the control group (P<0.01). But there is no significant difference between other groups and the control group (P>0.05). Also, ketamine recipient group with dose of 20 mg /kg + quercetin liposome with dose of 30 mg /kg, has significant difference with the Ethanol group (P<0.05). This group has a significant difference with the groups receiving ketamine (20 mg /kg)+ quercetin (30mg /kg) and quercetine liposome (30 mg /kg) (P<0.05). There is no significant difference between other groups (P>0.05). BDNF level: The analysis of the results shows that there is a significant difference between the group receiving ketamine (20 mg/kg) and the groups receiving ethanol and saline (10 mg/kg) at the level of P>0.05.TrkB level: The analysis of the results shows that there is no significant difference between the groups (P>0.05).The duration of time spent in the open arm on the first day: The analysis of the results shows that there is no significant difference between the groups (P>0.05).The time spent in the open arm on the seventh day: The analysis of the results shows that there is no significant difference between the groups (P>0.05).Percentage entering the open arm on day 1: The analysis of the results shows that there is a significant difference between the group receiving quercetin liposome (30 mg/kg) and the group receiving quercetin (30 mg/kg) (P<0.05). Percentage entering the open arm on day 7: The analysis of the results shows that there is no significant difference between the groups (P>0.05).Pyknotic Nuclei: Analysis of the results shows that the receiving groups Ketamine (20 mg/kg) and ketamine (20 mg/kg) + quercetin (30 mg/kg) have a very significant difference with the control group (receiver of saline) (10 mg/kg) (P<0.001). The groups receiving quercetin (30 mg/kg) and ketamine (20 mg/kg) + quercetin (30 mg/kg) have a significant difference from the group receiving ethanol (P < 0.01). The groups receiving quercetin (30 mg/kg) and ketamine (20 mg/kg) + quercetin liposome (30 mg/kg) and quercetin liposome (30 mg/kg) have a significant difference from the group receiving ketamine (20 mg/kg) (P<0 .001, P < 0.001, P < 0.05). The group receiving quercetin liposome (30 mg/kg) has a significant difference from the group receiving quercetin (30 mg/kg) (P < 0.05). The groups receiving quercetin liposome (30 mg/kg) and ketamine (20 mg/kg) + quercetin liposome (30 mg/kg), significantly different from the group receiving quercetin (30 mg/kg) + ketamine (20 mg) /kg) (P < 0.01, P < 0.001).Vasogenic Edema: Analysis of the results shows that the groups receiving quercetin (30 mg/kg) and ketamine (20 mg/kg) + quercetin (30 mg/kg) have a significant difference with the control group (receiver of saline) (10 mg/kg) (P < 0.05). The groups receiving quercetin (30 mg/kg) and quercetin liposome (30 mg/kg) have a significant difference with the ethanol group (P < 0.001). The groups receiving quercetin (30 mg/kg) and quercetin liposome (30 mg/kg) have a very significant difference with the group receiving ketamine (20 mg/kg) (P < 0.001). The groups receiving ketamine (20 mg/kg) + quercetin (30 mg/kg) and ketamine (20 mg/kg) + liposome quercetin (30 mg/kg) have a significant difference with the group receiving quercetin (30 mg/kg) (P < 0.001). The groups receiving quercetin liposome (30 mg/kg) and ketamine (20 mg/kg) + quercetin liposome (30 mg/kg) have a very significant difference with the group receiving ketamine (20 mg/kg) + quercetin liposome (30 mg) /kg) (P < 0.001).Inflammation: Analysis of the results shows that the groups receiving ketamine (20 mg/kg) and ketamine (20 mg/kg) + quercetin (30 mg/kg), have a significant difference with the control group (receiver of saline) (10 mg/kg) (P < 0.05, P < 0 .001). The groups receiving quercetin (30 mg/kg) and ketamine (20 mg/kg) + quercetin (30 mg/kg) and quercetin liposome (30 mg/kg) and quercetin (20 mg/kg) + quercetin liposome (30 mg/kg), have a significant difference with the group receiving ethanol (P < 0.01, P < 0.001, P < 0.01, P < 0.01). The groups receiving quercetin (30 mg/kg) and ketamine (20 mg/kg) + quercetin (30 mg/kg) and quercetin liposome (30 mg/kg) and quercetin (20 mg/kg) + quercetin liposome (30 mg/kg) , have a significant difference with the group receiving ketamine (20 mg/kg) (P < 0.001, P < 0.05, P < 0.001, P < 0.001). The group receiving ketamine (20 mg/kg) + quercetin (30 mg/kg) has a significant difference from the group receiving quercetin (30 mg/kg) (P < 0.001). The groups receiving ketamine (20 mg/kg) + quercetin liposome (30 mg/kg) and quercetin liposome (30 mg/kg), a significant difference with the group receiving ketamine (20 mg/kg) + quercetin (30 mg/kg) have (P < 0.001). Discussion: In general, the results show that by inducing schizophrenia by ketamine, anxiety states increase. In the treatment groups with antioxidant injection, these conditions improve. Regarding the parameters of oxidative stress, some of the results were contrary to expectations. A decrease in parameters was observed in the treatment groups. But in general, quercetin improves the condition. But in general, quercetin improves the condition.Keywords: Schizophrenia, elevated plus maze, BDNF, TrkB, oxidative stress, quercetin.

متن يادداشت

فاقد چکیده فارسی

عنوان گونه گون

بررسی تغییرات هیستولوژیکی و استریولوژیکی همراه با پارامترهای استرس اکسیداتیو و تغییرات بیان ژن های BDNF و TrkB در قشر پره فرونتال موش های صحرایی نر طی القای بیماری اسکیزوفرنی و درمان با لیپوزوم

اصطلاح موضوعی

Schizophrenia, elevated plus maze, BDNF, TrkB, oxidative stress, quercetin.

اصطلاح موضوعی

فاقد کلید واژه فارسی

عنصر شناسه اي

Barbar Obaid,

ساير عناصر نام

Afrah

کد نقش

Producer

عنصر شناسه اي

Hatami,

عنصر شناسه اي

Banan Khojasteh,

عنصر شناسه اي

Ahmadi,

ساير عناصر نام

Homeira

ساير عناصر نام

Seyed Mehdi

ساير عناصر نام

Hatam

کد نقش

Thesis advisor

کد نقش

Thesis advisor

کد نقش

Consulting advisor

عنصر شناسه اي

Tabriz

کشور

ایران

سازمان

Central Library University of Tabriz

تاريخ عمليات

20230522

شماره بازیابی

ارشد پایاننامه QL351.O33 1401

نام الکترونيکي

Afrah Barbar Obaid

ارتباط براي تسهيلات دسترسي

عبادی

فرمت انتشار

e

نوع ماده

TL

کد کاربرگه

276903

سطح دسترسي

a

تكميل شده

Y