U6 cm bove the liquid nitrogen surface) for 10 min before plunging into liquidnitrogen, for storage. The samples were evaluated for 150 days for parameters such as viability, motility and progressive motility and membrane integrity. To determine serum testosterone, blood samples were taken at a distance of zero, 30, 60 and 90 minutes after intramuscular injection of 500 IU hCG. To determine the seminal plasma testosterone,sperm sampling was done after zero, 1 and 7 days after injection of 500I