عنوان
پدید آورنده
موضوع
رده
کتابخانه
محل استقرار
شماره کتابشناسی ملی
شماره
TLpq304245223
زبان اثر
زبان متن نوشتاري يا گفتاري و مانند آن
انگلیسی
عنوان و نام پديدآور
عنوان اصلي
Importance of a siderophore in the pathogenesis and virulence of Photobacterium damsela subsp. piscicida in hybrid striped bass (Morone saxatili x Morone chrysops)
نام عام مواد
[Thesis]
نام نخستين پديدآور
J. P. Hawke
نام ساير پديدآوران
R. L. Thune
وضعیت نشر و پخش و غیره
نام ناشر، پخش کننده و غيره
Louisiana State University and Agricultural & Mechanical College
تاریخ نشرو بخش و غیره
1996
مشخصات ظاهری
نام خاص و کميت اثر
155
یادداشتهای مربوط به پایان نامه ها
جزئيات پايان نامه و نوع درجه آن
Ph.D.
کسي که مدرک را اعطا کرده
Louisiana State University and Agricultural & Mechanical College
امتياز متن
1996
یادداشتهای مربوط به خلاصه یا چکیده
متن يادداشت
Photobacterium damsela subsp. piscicida, formerly Pasteurella piscicida, is an important new pathogen of hybrid striped bass cultured in brackish water on Louisiana mariculture farms. Louisiana isolates compared with strains of P. damsela subsp. piscicida from Greece, Japan, Israel and Chesapeake Bay, USA and were found to be identical in biochemical phenotype and enzymic activity. Small plasmids of 8 and 5 kb were unique to Louisiana strains. Sequential daily bacteriology and histopathology on hybrid striped bass experimentally infected by immersion revealed that following high doses (13,000 CFU/ml) the gills and spleen are colonized first as early as 24 hours PI, followed by the blood at 48 hours and the liver at 72 hours. Bacteria reached levels as high as usd5.7\times10\sp9usd CFU/gram in the spleen and usd8.6\times10\sp8usd in the blood of moribund fish. The spleen contained a significantly higher number of cells than any other organ at all sampling time periods. Histological examination of the gills using semi-thin sections showed high numbers of bacteria invading and colonizing the gill lamellae at 24 hours PI. Death of the fish appeared to result from respiratory failure due to necrosis of the gill lamellae and blockage of blood flow to the gills by masses of bacteria in the capillaries. Pathogenic isolates of P. damsela subsp. piscicida were shown to produce an iron siderophore. A wild type strain 91-197, was mutagenized by introduction of a transposon on pEIS, a suicide delivery plasmid by electroporation. A stable mutant (LSU-P1) deficient in siderophore biosynthesis was generated using this system. LSU-P1 evaluated in an immersion infection model for hybrid striped bass and also by IP injection was found to be non-virulent. The insertion of the transposon into the genome of P. damsela subsp. piscicida in LSU-P1 was confirmed by PCR analysis using primers to the kan gene in the transposon.
موضوع (اسم عام یاعبارت اسمی عام)
موضوع مستند نشده
Aquaculture
موضوع مستند نشده
Biological sciences
موضوع مستند نشده
Fish production
موضوع مستند نشده
Microbiology
موضوع مستند نشده
Veterinary services
موضوع مستند نشده
Zoology
نام شخص به منزله سر شناسه - (مسئولیت معنوی درجه اول )
مستند نام اشخاص تاييد نشده
J. P. Hawke
مستند نام اشخاص تاييد نشده
R. L. Thune
دسترسی و محل الکترونیکی
نام الکترونيکي
مطالعه متن کتاب وضعیت انتشار
فرمت انتشار
p
اطلاعات رکورد کتابشناسی
نوع ماده
[Thesis]
کد کاربرگه
276903
اطلاعات دسترسی رکورد
سطح دسترسي
a
تكميل شده
Y
