عنوان

Amino acid analysis protocols

English

Amino acid analysis protocols

Totowa, N.J.

Humana Press

c2001

xi, 265 p. :ill. ;24 cm.

Methods in molecular biology ; 951

Includes bibliographical references and index.

edited by Catherine Cooper, Nicolle Packer and Keith Williams

1

Amino acid analysis: an overview -- Amino acid analysis, using postcolumn ninhydrin detection, in a biotechnology laboratory -- Purification of proteins using UltraMacro spin columns or ProSorb sample preparation cartidges for amino acid analysis -- Amino acid analysis using precolumn derivatization with 6-ami noquinolyl-N-hydroxysuccinimidyl carbamate -- Amino acid analysis by high-performance liquid chromatography after derivatization with 1-fluoro-2,4-dinitrophenyl-5-L-alanine amide)Marfey's Reagent( -- The analysis of amino acids using precolumn derivatizatin, HPLC, and electrochemical detection -- Anion exchange chromatography and intergrated amperometric detectin of amino acids -- Ion-pair chromatography for identification of picomolar-order protein on a PVDF membrane -- Capillary gas chromatographic analysis of protein and nonprotein amino acids in biological samples -- Measurement of blood plasma amino acids in ultrafiltrates by high-performance liquid chromatography with automatic precolumn o-phthaldialdehyde derivatization -- Determination of amino acids in foods by reversed-phase high-performance liquid chromatography with new precolumn derivatives, butylthiocarbamyl, and benzylthiocarbamyl derivatives compared to the phenylthiocarbamyl derivative and ion exchange chromatography -- Amino acid measurement in body fluids using PITC derivatives -- Determination of proteins, phosphatidylethanolamine, and phosphatidylserine in lipid-rich materials by analysis of phenylthiocarbamyl derivatives -- Analysis of o-phosphoamino acids in biological samples by gas chromatography with flame photometric detection -- Determination of sulfur amino acids, glutahione, and related aminothiols in biological samples by gas chromatography with flame photometric detection --- Capillary electrophoretic determination of 4-hydroxyproline -- Total plasma homocysteine analysis by HPLC with SBD-F precolumn derivatization -- Determination of early glycation products by mass spectrometry and quantification of glycation mediated protein crosslinks by the incorporation of ]p41sC[lysine into proteins.

، Amino acids - Analysis

TI

AU Cooper, Catherine 1970-

AU Packer, Nicolle.

AU Williams, Keith 1948-

SE