عنوان
پدید آورنده
موضوع
رده
کتابخانه
محل استقرار
IR
۳۴۱پ
فارسی
IR
بررسی نقش فاکتور رونویسی CREB1 در مسیر القایی هیدروکسی اوره در القای ژن گاماگلوبین
[پایان‌نامه]
Investigating the Role of CREB1Transcription Factor in Hydroxyurea-Mediated -goblin Induction Pathway
علوم بهزیستی وتوانبخشی university of social welfare and rehabilitation))
، ۱۳۸۹
۱۰۶ص.
چاپی
کارشناسی ارشد
ژنتیک genetic
علوم بهزیستی وتوانبخشی university of social welfare and rehabilitation))
promoter.Key words:HU. CREB1 . siRNA .ARMSPCR, EMSA عglobin induction pathway. This induction may in part be mediated via the Creb1 binding sites in the G- ع /promoter.Discussion: These results suggest that Creb1 is required for the HU عexpression in K562 cells. EMSAs show that HU increases levels of Creb1 complexes binding to the G عand A عglobin induction in K562 cells. qARMS-PCR results suggest that HU induces both G -ع promoter.Results: We have determined that knockdown of Creb1 blocks HU-mediated عtranscript levels. We have used EMSA to investigate levels of Creb1-complex attachment to the G عand A عglobin up regulation. We have set up a quantitative ARMS-PCR approach to differentiate between G-ع expression through a Creb1/ATF-2 binding site present exclusively in the promoter of Methods:Here we have used an (RNAi) approach to investigate the role of Creb1 in HU-mediated عvia p38 MAPK phosphorylation. It has been shown that SB induces G ع( induction in erythroid cells. In addition, both HU and SB activate Gع)and not A عglobin induction signaling pathway. First, both HU and SB lead to G-ع/globin induction is not completely understood. Similarities between the signaling pathways of hydroxyurea (HU) and sodium butyrate (SB) suggest involvement of Creb1 in the downstream part of the HU-ع Introduction: Hydroxyurea is commonly used for thalassemia treatment. However its signaling pathway leading to
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Investigating the Role of CREB1Transcription Factor in Hydroxyurea-Mediated -goblin Induction Pathway
HU
HU
HU
اسماعیل زاده، الیکا
Esmaeilzadeh, Elika
بنان، مهدی
نجم آبادی، حسین
ایران
دانشگاه علوم توان بخشی و سلامت اجتماعی
۳۴۱پ
341..pdf
محرمانه
محرمانه
3411.pdf
2304538
مرجع
متن
0
۱۴۳پایا
محل نگهداری: دانشگاه علوم بهزیستی و توانبخشی
نمایه‌سازی قبلی
چاپی-الکترونیکی
TF
1
a
Y
