عنوان

ارزیابی ایمنی متقاطع واکسن تری والان سونیکه کلی باسیلوزطیوردرجوجه های گوشتی

‭۱۳۶۲۸پ‬

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ارزیابی ایمنی متقاطع واکسن تری والان سونیکه کلی باسیلوزطیوردرجوجه های گوشتی

: دامپزشکی

، راشدی

چاپی

دکتری

دامپزشکی

‮‭۱۳۹۴/۰۶/۱۷‬

تبریز

کلی اسیلوز یکی از مهمترین بیماری های باکتریایی است که صنعت طیور در تمام دنیا درگیر آن می‌ب اشد و در ایران نیز بسیار شایع است و با ایجاد تلفات، کاهش رشد و کاهش کیفیت گوشت خسارات سنگینی را به صنعت مرغداری و کشور وارد می‌صکند .درمان این بیماری از طریق تجویز آنتی‌صبیوتیک‌صها صورت می‌صگیرد که علاوه بر تحمیل هزینه‌صهای سنگین به تولیدکنندگان باعث افزایش مقاومت میکربی و کاهش بهره برداری از پرورش طیور و کیفیت گوشت نیز می‌صگردد .یکی از راه‌صهای موثردر پیشگیری از کلی‌صباسیلوز، واکسیناسیون می‌صباشد .در این مطالعه از مخلوط سه سروتیپ‮‭O۷۸:K۸۰‬ ، ‮‭O۲:K۱‬ و ‮‭O۱:K۱‬ اشریشیاصکلی سونیکه به همراه ادجوانت آلوم واکسن تهیه و در ‮‭۹۶‬ قطعه جوجه گوشتی، ایمنی متقاطع آن مورد مطالعه قرار گرفت .در این مطالعه واکسن مورد نظر از مخلوط سه سروتیپ‮‭O۷۸:K۸۰‬ ، ‮‭O۲:K۱‬ و ‮‭O۱:K۱‬ اشریشیاصکلی) غیرفعال شده بوسیله اولتراسونیک (و با افزودن ادجوانت آلوم به عنوان ماده کمکی تهیه شد .جهت ارزیابی ایمنی‌صزایی واکسن تهیه شده، تعداد ‮‭۹۶‬ قطعه جوجه گوشتی نژاد راس انتخاب و بصورت کاملا تصادفی به چهار گروه شامل ‮‭۱)‬ گروه واکسینه چالش داده شده با‮‭O۲۶‬

O2:K1 and O1:K1 Abstract continue Escherichia Coli along with adjuvant Alum the vaccine is prepared and studied in 96 chickens for their cross immunity. In order to evaluate the immunity of the vaccine, 96 broiler chickens were selected and randomly were divided to four groups: 1. The vaccinated group which challenged with O26 strain. 2. The vaccinated group which challenged with O78 strain. 3. The non-vaccinated group which challenged with O26 strain.4.The non-vaccinated group which challenged with O78 strain. When the chickens were 14 days old, 0.5 cc vaccine and normal saline were injected at subcutaneous dorsal neck. When they were 35 days old, all groups were challenged as mentioned above. For serologic study, bleeding were weekly carried out from the vessel of the wing in all groups before and after vaccination and challenge. For evaluation the efficacy of the vaccines, in addition of serologic studies, the amount of weight gain, food consumption, FCR, clinical symptoms, and other parameters were recorded. After challenging, typical symptoms of Colibacillosis such as severe reduction of appetite, average loss of weight gain were observed in the non-vaccinated groups. Increase in weight gain of vaccinated group which was challenged with O78 was more than other groups and final weight of vaccinated group which was challenged with O26 was more than other groups. Food change factor in vaccinated groups was less than non-vaccinated groups. According to the results of this study, it seems that the vaccine was able to develop immunity against O78 and O26 serogroups, but immunization against O78 is better than O26. Finally, it can be concluded that the cross-protective immunity exists but it is not the same level of Homologous immunity ،O2:K1 and O1:K1 Escherichia Coli (inactivated using ultrasonic method) and after adding Alum as auxiliary material it is prepared. In this study, through combining three serotypes of O78:K80 ،Colibacillosis is one of the most important bacterial disease that all poultry industry deals with it all over the world. This disease is regularly observed in Iran and causes sever economic losses, reduction of growth and reduction of meat quality in the poultry industry. Antibiotic are usually used to treat diseased birds that in addition to high expenses for the producers, causes bacterial resistance and reduction of meat quality. Since there is not suitable and complete method for Control of the susceptible agents, especially respiratory disease, thus, one of the effective methods for prevention is vaccination. In this study, the vaccine is produced by combining three serotypes of O78:K80

عالم پوررجبی،سیما، پژوهشگر

رجبی، ذوالفقار، استادراهنما

جعفری، یاسر، استادمشاور

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