۴۱۶۲پ
per
بررسی چند شکلی ژنIGFBP - ۳به روشRFLP - PCRدر گاوهای هلشتاین و ارتباط آن با برخی از صفات تولیدی
/ساناز حسین پور سرای
دانشگاه تبریز: دانشکده کشاورزی، گروه علوم دامی
۵۰ص
زبان چکیده: استادراهنما مقدم، غلامعلی
چاپی
فاقد اطلاعات کامل
کارشناسی ارشد
علوم دامی
۱۳۸۹/۰۶/۲۵
دانشگاه تبریز: دانشکده کشاورزی، گروه علوم دامی
.Insulin- like growth factors (IGF-I, II) are necessary for growth, development and metabolic activities of mammals, and mediate most effects of the growth hormones. Their biological activities are inhibited by binding proteins, IGFBP-s which in turn inhibits growth of cells. Different types of IGFBP-s differ in their molecular weight, location effect of their amino- acids, distribution in biological liquid and their effect on IGF activity. The objective of this study was to detect of polymorphism in IGFBP-۳ gene of the Holstein cows and it's relation to productive traits. To achieve this, blood samples were collected from ۱۰۰ Holstein cows in Dashet- e- Azarnegin dairy farm which their pedigree as well as required records were available. After DNA extraction, the PCR- RFLP technique followed by amplifying a fragment of the gene (۳۱۶ bp in length) using specific primers. Then the PCR product was digestive using restriction enzymes HaeIII and BfaI and finally electrophoresis was done on ۲ agaros gel. The digestion of PCR products with each enzyme, produced four fragments sized ۱۹۵, ۶۹, ۴۴, and ۸ bp respectively which were similar in all of studied animals. However, in this study, one genotype was observed in studied farm
حسین پور سرای، ساناز
پیرانی، نصرالله، استاد راهنما
مقدم، غلامعلی، استاد مشاور
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