عنوان

اندازه گیری دفراسیروکس و بوتالکس با استفاده از روش فلوئورسانس حساس شده ی تربیوم

‭۱۶۲۳پ‬

per

اندازه گیری دفراسیروکس و بوتالکس با استفاده از روش فلوئورسانس حساس شده ی تربیوم

/وحید پناهی آذر

تبریز :دانشگاه تبریز

‮‭۱۰۹‬ص.‬

:مصور،جدول،نمودار‮‭۳۰‬*‮‭۲۹‬س.م- +لوح فشرده

چاپی

واژه‌نامه بصورت زیرنویس

کتابنامه ص.:‮‭۹۸-۱۰۶‬

کارشناسی ارشد

شیمی تجزیه

‮‭۱۳۸۸/۰۶/۲۵‬

تبریز :دانشگاه تبریز

In this thesis we determined deferasirox and butalax with direct and indirect method, respectively.Section I: Determination of deferasirox with direct method novel, rapid and sensitive spectroflurimetric method was developed and validated for the determination of deferasirox in urine, serum and tablet samples based on sensitization of terbium fluorescence. The excitation and emission wavelengths were 328 and 545 nm, respectively. The optimum conditions for the determination of deferasirox were investigated considering the effects of various affecting parameters. The method was quantitatively evaluated in terms of linearity, recovery, reproducibility and limit of detection. Under the optimal conditions, the fluorescence intensities were linear with the concentration of deferasirox in the range of 5-10-9 - 5-10-6 M, with a detection limit of 1.5-10-9 M and the relative standard deviations of 1.1 - 2.3 . Linearity, reproducibility, recovery and limit of detection made the method suitable for determination of deferasirox in urine, serum and tablets samples.Section II: Determination of butalax with indirect method selective and sensitive luminescence method for the determination of butalax is described. A new spectro?uorimetric method is reported for the determination of butalax, based on its quenching effect on the fluorescence intensity of Tb3+-deferasirox complex as a ?uorescent probe. The quenching of the terbium fluorescence due to the complex formation was quantitative for the butalax. The experiments indicated that the fluorescence intensity of the Tb3+- butalax was greatly enhanced by the deferasirox. On the basis of the above findings, a sensitive fluorimetric method for determining the butalax was established. The excitation and emission wavelengths were 328 and 545 nm, respectively. The optimum conditions for the determination of butalax were investigated considering the effects of various affecting parameters. The quenched ?uorescence intensity is proportional to the concentration of butalax. The enhanced fluorescence intensity of the system (?F ) showed a good linear relationship with the concentration of butalax in the range of 0.01-1.5 mg/L, its correlation coefficient was 0.999 and the detection limit (S/N=3) was 0.003 mg/L. The presented method was used to determine the butalax in real pharmaceutical samples. Linearity, reproducibility, recovery and limit of detection made the method suitable for determination of butalax in urine, serum, milk, cheese, meat, cream and tablets samples.

Terbium-sensitized

deferasirox

پناهی آذر،وحید

منظوری لشکر،جمشید، استاد راهنما

جویبان،ابوالقاسم، استاد راهنمای همکار

امجدی،محمد، استاد مشاور

سیاه و سفید

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