مکان یابی ژن (های) کنترل کننده عملکرد دانه و برخی صفات فیزیولوژیک درگندم نان تحت تنش خشکی با نشانگرهای ریز ماهواره
/محمد سخنور
: کشاورزی
۱۰۰ ص
جدول، نمودار،عکس
چاپی
واژه نامه بصورت زیرنویس
کتابنامه ص.: ۸۶-۹۷
کارشناسی ارشد
مهندسی کشاورزی-بیوتکنولوژی
۱۳۸۹/۰۶/۲۵
تبریز
.Drought stress is one of most important abiotic stress affecting growth and production of crop plants. To map gene(s) controlling traits related to drought tolerance in wheat, a recombinant inbred lines population derived from a cross between Azar2 (drought tolerant) and 87zhong291 (drought sensitive) was evaluated under water rain feed and supplementary irrigation. The measured traits were stomatal resistance, leaf temperature, leaf relative humidity, grain yield, 1000 grain weight and number of spikelet per spike. Parental polymorphism was assessed using microsatellite and ISSR markers and 24 SSR marker and 16 ISSR polymorphic markers were used to screen population individuals. Linkage analysis was performed to assign these markers to the population linkage map consisted of 45 SSR and AFLP markers. Since agronomical and physiologic traits were measures on 142 and 121 individuals of the populations, respectively, therefore two linkage maps containing 142 and 121 individuals were constructed. The first linkage map span 1310 cM of wheat genome with 19.55 cM distance between two neighboring markers. The length of second map was 1157.1 cM with 17.57 cM distance between two neighboring markers. Composite interval mapping was used to analysis association between genotypic and phenotypic data. Eighty four and 14 QTLs were identified for agronomic and physiologic traits, respectively. For grain yield, 10 and 20 QTLs were detected under rain feed and supplementary irrigation, respectively. One, two and 11 QTLs were mapped for leaf relative humidity, stomatal resistance and leaf temperature, respectively. Under rain feed condition, five and supplementary irrigation nine QTLs were identified for number of spikelet per spike. For 1000 grain weight, two QTLs under rain feed condition and nine QTLs under supplementary irrigation were detected. In the present study, one and three stable QTLs at rain feed and supplementary irrigation conditions were observed grain yield and 1000 grain weight, respectively. Some QTLs were common for 1000 grain weight, number of spikelet per spike, leaf temperature and grain yield which could be due to gene linkage or plieotropic effect. Some of The markers identified in this study tightly linked to QTLs could be used to selection of drought tolerant lines.Keywords: wheat, drought, physiological traits, composite interval mapping, SSR and ISSR markers, QTL