۲۲۹۱۶پ
per
اثر عصاره متانولی دانه آووکادو بر ساختار هیستولوژیک و استریولوژیک بافت کلیه در موش سوری مبتلا به دیابت نوعII
Effect of methanolic extract of Avocado (Persea americana) seed on histological and stereological structure of kidney in mice with diabetes type II
/امیر ممی پور
: دامپزشکی
، ۱۳۹۷
، راشدی
۱۱۴ص
چاپی - الکترونیکی
کارشناسی ارشد
دامپزشکی
۱۳۹۷/۰۶/۲۰
تبریز
Type II diabetes is a metabolic disorder caused by metabolic imbalances that can gradually disrupts the function of many organs such as kidneys, and its global prevalence has been increasing markedly over the last decade. Perevious studies have shown that many diabetic patients, along with blood glucose control drugs, use other supportive supplementary materials such as herbal extracts. Avocado (Persea americana) is a native tree of Central and South America that has traditionally been used due to its antioxidant, anti-inflammatory, antihyperglycemic, anti-hyperlipidemic and anti-hypertensive effect but there is no scientific information about effect of Avocado seed extract on histological and stereological structure of kidney and also its protective effects on diabetes type II side effect in kdiney. Therefore, this study was conducted to investigate the effect of methanolic extract of Avocado (Persea americana) seed on histological and stereological structure of kidney in mice with diabetes type I by stereological methods. In this study, 40 adult male mice were randomly divided in to four groups of control and four groups of diabetes. Diabetes was induced by high fat diet plus dose 150 mg/kg of streptozotocin injection. Control and diabetic groups received 0, 50, 100 and 150 mg/kg methanolic extract of Avocado seed via oral gavage for 40 consecutive days respectively. Blood glucose level was measured during experimental period. At the end of experiment, animals sacrificed by cervical dislocation, and their left and right kidneys were removed. The left kidney was fixed in buffered formalin 10 , processed by standard and routine paraffin embedding. Sections were stained by H&E and histological and stereological studies were performed. Unbiased stereological methods have been used to estimate total volume of left kidney (cortex and medulla regions), volume of renal corpuscle (urinary space and glomerulus), volume, length, diameter and epithelium height of poroximal and distal tubules. Total number of glomerulus was estimated by optical dissector. Finally, one-way ANOVA and Tukey's post hoc test were performed to statistical analysis for comparison between groups. The results showed that induction of diabetes cased to significant increasing of blood glucose and insulin level and reduction of body weight, kidney weight and total volume but did not change the relative weight of the kidney. Microscopic study of the kidney structure indicated that diabeted induced structural changes in cortex (decrease in size of renal corpuscle and glomerulus, as well as destruction of epithelium of proximal and distal tubules) and medullae (increasing of interstitial tissue and vascular hyperemia). Stereological studies revealed that diabetes induction significantly reduced total volume of kidney, renal cortex, corpuscle and glomerulus, epithelium of proximal and distal tubules and total number of renal corpuscle, and also increased total volume of renal capsule, interstitial tissue, urinary space and lumen of proximal tubules and caused structural destruction of kidney but methanolic extract of Avocado seed could reduce blood glucose leve and increase insulin level and prevent the diabetes induced structural complications of kidney in a dose dependent manner. Based on the results of this study, it can be concluded that methanolic extract of Avocado seed can be prevented adequately from renal damage cased by type II diabetes and improve the kidney structure in diabetic patients. Therefore it can be considered as an appropriate therapeutic strategy for improving kidney function in diabetics
Effect of methanolic extract of Avocado (Persea americana) seed on histological and stereological structure of kidney in mice with diabetes type II
ممی پور، امیر
Mamipour, Amir
سیاه و سفید
نمایهسازی قبلی
