What is synthetic biology? /Karen M. Polizzi --Gene synthesis method based on overlap extension PCR and DNAWorks program /Gang Li ... [et al.] --BioBrick[TM] assembly using the in-fusion PCR cloning kit /Sean C. Sleight and Herbert M. Sauro --Overlap extension PCR cloning /Anton Bryksin and Ichiro Matsumura --One-step isothermal assembly of DNA fragments /Rui T.L. Rodrigues and Travis S. Bayer --Creation and characterization of component libraries for synthetic biology /Tim Weenink and Tom Ellis --In vivo and in vitro characterization of [sigma]a?a? constitutive promoters by real-time PCR and fluorescent measurements /James Chappell and Paul Freemont --In vivo screening of artificial small RNAs for silencing endogenous genes in Escherichia coli /Vandana Sharma and Yohei Yokobayashi --Construction and engineering of large biochemical pathways via DNA assembler /Zengyi Shao and Huimin Zhao --Assembly of multi-gene pathways and combinatorial pathway libraries through ePathBrick vectors /Peng Xu and Mattheos A.G. Koffas --Tandem recombineering by SLIC cloning and Cre-LoxP fusion to generate multigene expression constructs for protein complex research /Matthias Haffke ... [et al.] --Combinatorial DNA assembly using Golden Gate cloning /Carola Engler and Sylvestre Marillonnet --Construction of synthetic gene circuits in the Escherichia coli genome /Bei-Wen Ying, Yuya Akeno, and Tetsuya Yomo --Shuffling of DNA cassettes in a synthetic integron /David Bikard and Didier Mazel --Systematic methodology for the development of mathematical models for biological processes /Cleo Kontoravdi --SOBOLHDMR : a general-purpose modeling software /Sergei Kucherenko.
Methods in molecular biology (Clifton, N.J.) ;v. 1073.1064-3745