Methods for sperm concentration determination /Lars Bjorndahl --Methods of sperm vitality assessment /Sergey I. Moskovtsev and Clifford L. Librach --Hypo-osmotic swelling test for evaluation of sperm membrane integrity /Sivakumar Ramu and Rajasingam S. Jeyendran --Sperm morphology classification : a rational method for schemes adopted by the World Health Organization /Susan A. Rothmann ... [et al.] --Sperm morphology assessment using strict (tygerberg) criteria /Roelof Menkveld --Methods for direct and indirect antisperm antibody testing /Hiroaki Shibahara and Junko Koriyama --Manual methods for sperm motility assessment /David Mortimer and Sharon T. Mortimer --Computer-aided sperm analysis (CASA) of sperm motility and hyperactivation /David Mortimer and Sharon T. Mortimer --Hemizona assay for assessment of sperm function /Sergio Oehninger, Mahmood Morshedi, and Daniel Franken --Sperm penetration assay for the assessment of fertilization capacity /Kathleen Hwang and Dolores J. Lamb --Methods for the assessment of sperm capacitation and acrosome reaction excluding the sperm penetration assay /Christopher J. De Jonge and Christopher L.R. Barratt --Sperm DNA fragmentation analysis using the TUNEL assay /Rakesh Sharma, Jayson Masaki, and Ashok Agarwal --Sperm DNA damage measured by comet assay /Luke Simon and Douglas T. Carrell --Sperm chromatin structure assay (SCSA) /Donald P. Evenson --Sperm aneuploidy testing using fluorescence in situ hybridization /Benjamin R. Emery --Flow cytometric methods for sperm assessment /Vanesa Robles and Felipe Martinez-Pastor --Human y chromosome microdeletion analysis by PCR multiplex protocols identifying only clinically relevant AZF microdeletions /Peter H. Vogt and Ulrike Bender --Sperm cryopreservation methods /Tiffany Justice and Greg Christensen --Density gradient separation of sperm for artificial insemination /David Mortimer and Sharon T. Mortimer --Recovery, isolation, identification, and preparation of spermatozoa from human testis /Charles H. Muller and Erin R. Pagel --Enhancement of sperm motility using pentoxifylline and platelet-activating factor /Shilo L. Archer and William E. Roudebush --Intracytoplasmic morphology-selected sperm injection /Luke Simon, Aaron Wilcox, and Douglas T. Carrell --Sperm selection for ICSI using annexin V /Sonja Grunewald and Uwe Paasch --Sperm selection for ICSI using the hyaluronic acid binding assay /Mohammad Hossein Nasr-Esfahani and Tavalaee Marziyeh --sperm selection based on electrostatic charge /Luke Simon, Shao-qin Ge, and Douglas T. Carrell --Sex-sorting sperm using flow cytometry/cell sorting /Duane L. Garner, K. Michael Evans, and George E. Seidel --Assessment of spermatogenesis through staging of seminiferous tubules /Marvin L. Meistrich and Rex A. Hess --Immunohistochemical approaches for the study of spermatogenesis /Cathryn A. Hogarth and Michael D. Griswold --Ultrastructural analysis of testicular tissue and sperm by transmission and scanning electron microscopy /Hector E. Chemes --Assessment of oxidative stress in sperm and semen /Anthony H. Kashou, Rakesh Sharma, and Ashok Agarwal --Improved chemiluminescence assay for measuring antioxidant capacity of seminal plasma /Charles H. Muller, Tiffany K.Y. Lee, and Michalina A. Montano --Methods of sperm DNA extraction for genetic and epigenetic studies /Jeanine Griffin --Isolating mRNA and small noncoding RNAs from human sperm /Robert J. Goodrich, Ester Anton, and Stephen A. Krawetz --Review of genome-wide approaches to study the genetic basis for spermatogenic defects /Kenneth I. Aston and Donald F. Conrad --Methods for the analysis of the sperm proteome /Sara de Mateo, Josep Maria Estanyol, and Rafael Oliva --Methodology of aniline blue staining of chromatin and the assessment of the associated nuclear and cytoplasmic attributes in human sperm /Leyla Sati and Gabor Huszar --Isolation of sperm nuclei and nuclear matrices from the mouse, and other Rodents /W. Steven Ward --Protamine extraction and analysis of human sperm protamine 1/protamine 2 ratio using acid gel electrophoresis /Lihua Liu, Kenneth I. Aston, and Douglas T. Carrell --Analysis of gene-specific and genome-wide sperm DNA methylation /Saher Sue Hammoud, Bradley R. Cairns, and Douglas T. Carrell --Evaluating the localization and DNA binding complexity of histones in mature sperm /David Miller and Agnieszka Paradowska --In vitro spermatogenesis using an organ culture technique /Tetsuhiro Yokonishi ... [et al.] --Testicular tissue grafting and male germ cell transplantation /Jose R. Rodriguez-Sosa, Lin Tang, and Ina Dobrinski --Transgenic modification of spermatogonial stem cells using lentiviral vectors /Christina Tenenhaus Dann --Methods for sperm-mediated gene transfer /Marialuisa Lavitrano, Roberto Giovannoni, and Maria Grazia Cerrito --Phenotypic sssessment of male fertility status in transgenic animal models /David M. de Kretser and Liza O'Donnell.
Methods in molecular biology (Clifton, N.J.) ;v. 927.1064-3745