عنوان

Neural stem cells

0896039641

IR

EB7478

انگلیسی

IR

Neural stem cells

[Electronic Resource]

:methods and protocols

/ edited by Tanja Zigova, Paul R. Sanberg, Juan R. Sanchez-Ramos

Totowa, NJ

: Humana Press,

, c2002.

xiv, 381 p. ill. 24 cm.

(Methods in molecular biology

; 198)

e

ng

Includes bibliographical references and index.

Machine generated contents note: Prefacev -- Contributors xi -- PART I ISOLATION AND CULTURE OF NSCs -- 1 Neural Differentiation of Embryonic Stem Cells -- K. Sue O'Shea-- 2 Production and Analysis of Neurospheres from Acutely Dissociated and Postmortem CNS Specimens -- Eric D. Laywell, Valery G. Kukekov, Oleg Suslov, -- Tong Zheng, and Dennis A. Steindler 15 -- 3 Isolation of Stem and Precursor Cells from Fetal Tissue -- Yuan Y. Wu, Tahmina Mujtaba, and Mahendra S. Rao29 -- 4 Olfactory Ensheathing Cells: Isolation and Culture from the Rat Olfactory Bulb -- Susan C. Barnett and A. Jane Roskams 41 -- 5 Culturing Olfactory Ensheathing Glia from the Mouse Olfactory Epithelium -- Edmund Au and A. Jane Roskams49 -- 6 Production of Immortalized Human Neural Crest Stem Cells -- Seung U. Kim, Eiji Nakagawa, Kozo Hatori, Atsushi Nagai, -- Myung A. Lee, and Jung H. Bang55 -- 7 Adult Rodent Spinal Cord Derived Neural Stem Cells: Isolation and Characterization -- Lamya S. Shihabuddin67 -- 8 Preparation of Neural Progenitors from Bone Marrow and Umbilical Cord Blood -- Shijie Song and J. Sanchez-Ramos79 -- 9 Seeding Neural Stem Cells on Scaffolds of PGA, PLA, and Their Copolymers -- Erin Lavik, Yang D. Teng, Evan Snyder, and Robert Langer 89 -- PART II CHARACTERIZATION OF NSCs IN VITRO -- A. CELLULAR TECHNIQUES -- 10 Analysis of Cell Generation in the Telencephalic Neuroepithelium -- Takao Takahashi, Verne S. Caviness, Jr., -- and Pradeep G. Bhide101 -- 11 Clonal Analyses and Cryopreservation -- of Neural Stem Cell Cultures -- Angelo L. Vescovi, Rossella Galli, and Angela Gritti115 -- 12 Assessing the Involvement of Telomerase in Stem Cell Biology -- Mark P. Mattson, Peisu Zhang, and Weiming Fu 125 -- 13 Detection of Telomerase Activity in Neural Cells -- Karen R. Prowse137 -- 14 In Vitro Assays for Neural Stem Cell Differentiation -- Marcel M. Daadi 149 -- 15 Electron Microscopy and Lac-Z Labeling -- Bela Kosaras and Evan Snyder157 -- B. ELECTROPHYSIOLOGICAL TECHNIQUES -- 16 Techniques for Studying the Electrophysiology of Neurons Derived from Neural Stem/Progenitor Cells -- David S. K. Magnuson and Dante J. Morassutti179 -- C. MOLECULAR TECHNIQUES -- 17 Fluorescence In Situ Hybridization -- Barbara A. Tate and Rachel L. Ostroff 189 -- 18 RT-PCR Analyses of Differential Gene Expression -- in ES-Derived Neural Stem Cells -- Theresa E. Gratsch197 -- 19 Differential Display: Isolation of Novel Genes -- Theresa E. Gratsch213 -- 20 Cell Labeling and Gene Misexpression by Electroporation -- Terence J. Van Raay and Michael R. Stark223 -- 21 Gene Therapy Using Neural Stem Cells -- Luciano Conti and Elena Cattaneo 233 -- 22 Modeling Brain Pathologies Using Neural Stem Cells -- Simonetta Siplone and Elena Cattaneo245 -- PART III UTILIZATION/CHARACTERIZATION OF NSCs IN VivO -- A. ENDOGENOUS POOLS OF STEM/PROGENITOR CELLS -- 23 Activation and Differentiation of Endogenous Neural Stem Cell Progeny in the Rat Parkinson Animal Model -- Marcel M. Daadi 265 -- 24 Identification of Musashil-Positive Cells in Human Normal and Neoplastic Neuroepithelial Tissues by Immunohistochemical Methods -- Yonehiro Kanemura, Shin-ichi Sakakibara, -- and Hideyuki Okano273 -- 25 Identification of Newborn Cells by BrdU Labeling and Immunocytochemistry In Vivo -- Sanjay S. P. Magavi and Jeffrey D. Macklis283 -- 26 Immunocytochemical Analysis of Neuronal Differentiation -- Sanjay S. P. Magavi and Jeffrey D. Macklis291 -- 27 Neuroanatomical Tracing of Neuronal Projections with Fluoro-Gold -- Lisa A. Catapano, Sanjay S. P. Magavi, -- and Jeffrey D. Macklis299 -- B. TRANSPLANTATION -- 28 Labeling Stem Cells In Vitro for Identification of Their Differentiated Phenotypes After Grafting into the CNS -- Qi-lin Cao, Stephen M. Onifer, and Scott R. Whittemore307 -- 29 Optimizing Stem Cell Grafting into the CNS -- Scott R. Whittemore, Y. Ping Zhang, Christopher B. Shields, -- Dante J. Morassutti, and David S. K. Magnuson319 -- 30 Vision-Guided Technique for Cell Transplantation and Injection of Active Molecules into Rat and Mouse Embryos -- Lorenzo M agrassi327 -- 31 Transplantation into Neonatal Rat Brain as a Tool to Study Properties of Stem Cells -- Tanja Zigova and Mary B. Newman341 -- 32 Routes of Stem Cell Administration in the Adult Rodent -- Alison E. Willing, Svitlana Garbuzova-Davis, -- Paul R. Sanberg, and Samuel Saporta357 -- Index3 7 5.

Methods in molecular biology (Clifton, N.J.)

v. 198

Neural stem cells Laboratory manuals

Zigova, Tanja

Sanberg, Paul R

Sanchez-Ramos, Juan Raymond,1945-

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محرمانه

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old catalog

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