Physicochemical properties of drugs and metabolites and their impact on bioanalysis / Hugh Wiltshire and Angus Nedderman -- Sample preparation / Christopher A. James -- Basic HPLC theory and pracice / Andy Gray and Peter Lawrence -- HPLC optimisation / David Bakes -- HPLC detectors / Kenneth C. Saunders and Richard F. Venn -- Gas chromatography: what it is and how we use it / Peter Andrew and Mira V. Doig -- Thin-layer chromatography / Hugh Wiltshire and Angus Nedderman -- Immunoassay techniques / Richard F. Venn -- Automation of sample preparation / Kenneth C. Saunders and Christopher A. James -- Fundamental aspects of mass spectrometry / Mira V. Doig and David M. Higton -- Quantitative LC-MS / David M. Higton and Mira V. Doig -- Mass spectrometric identification of metabolites / Gordon Dear -- Nuclear magnetic resonance in drug metabolism / Phil Gilbert -- Metabolite isolation / Gordon Dear, Angus Neddermann, and Hugh Wiltshire -- Strategy for the development of quantitative bioanalytical procedures / David Bakes.