عنوان

Cloning and expression of calpain and phytase genes for the improvement of animal growth and nutrition

TLpq304261593

انگلیسی

Cloning and expression of calpain and phytase genes for the improvement of animal growth and nutrition

[Thesis]

W. Sun

D. L. M. Hancock, William M.; Patterson, J. A.

Purdue University

1996

184

Ph.D.

Purdue University

1996

The calpain system is a critical protease system in protein accretion and animal muscle growth. The calpain cDNA's from pigs and cattle were cloned to study the roles of calpain in muscle growth in domestic animals. By using reverse transcriptase and polymerase chain reaction, porcine mu- and m-calpain, and bovine mu-, m-, skeletal muscle specific (skm) calpain, and calpastatin cDNA's were cloned. Sequence analysis indicated that porcine and bovine calpain sequences were about 90% identical to rabbit or rat calpain sequences. The transcriptional sizes of porcine and bovine calpains were 3.0 kb for mu-calpain; 3.1 and 1.5 kb for m-calpain; 3.9 kb for skm-calpain; 3.8 and 5.0 kb for calpastatin. The expression of calpain genes were then studied in the steers treated with a usd\betausd-agonist, cimaterol. The expression of mu-, m-calpain and skm-calpain genes and activities were not changed by the cimaterol treatment. However, calpastatin mRNA and activity were increased by cimaterol (p < 0.05), and calpastatin mRNA and the rate of protein accretion were highly correlated (pusd