Biodegradation of dodecane by indigenous microorganisms
[Thesis]
M. Y. A. Miah
R. N. Finch
Texas A&M University - Kingsville
1996
133
M.S.
Texas A&M University - Kingsville
1996
Contamination of soil by hazardous substances has the potential for a major impact on the public health and balance of the ecosystem. Petroleum hydrocarbons are global environmental pollutants. Dodecane is one of the major ingredients of jet fuels and other light mineral oils. The main objective of this study involves the determination of the rate of biodegradation of dodecane and the evaluation of the best conditions to achieve a maximum degradation of dodecane by indigenous microorganisms in soil within the experimental design considered. The environmental parameters assessed for achieving the best degradation rate were moisture content, temperature and substrate (dodecane) concentration. A Comput-OX WB 600 series respirometer was utilized to perform all of the experiments. Kinetic parameters were estimated by using the method of Least Squares. First order kinetics was observed in all the of experiments. The maximum degradation was observed at 19 Wt% moisture, 33C and 2 Wt% substrate (dodecane) for the experimental design considered. The average rates constant were calculated to be 0.0534 day, 0.0629 day and 0.0224 day, respectively, for 1 Wt%, 2 Wt%, and 3 Wt% of dodecane concentrations.