عنوان

Fenolik asitlerin varlığında patojen bakterilerin moleküler incelemesi

TLpq2524198237

انگلیسی

Fenolik asitlerin varlığında patojen bakterilerin moleküler incelemesi

[Thesis]

Özdemir, Özgün Öykü

Soyer, Ferda

Izmir Institute of Technology (Turkey)

2019

236

Ph.D.

Izmir Institute of Technology (Turkey)

2019

Pathogenic bacteria, including P. aeruginosa, are serious threats for human health with their antibiotic resistance and virulence factors. Since phenolic acids, secondary metabolites of plants, can be good candidates as antimicrobial agents, their mode of action should be investigated. Proteomics is one of the main approaches for elucidating the mode of action of such compounds. In addition, in order to enhance the antimicrobial effects and stabilities of phenolics, they can be encapsulated into nanoparticles. The nanoparticles can be produced from chitosan and alginate which are biocompatible polymers. In this study, the antimicrobial effects of 3-HPAA and 4-HBA were presented with MIC values of 2.1 and 1.9 mg/ml, respectively. The bacteriocidal effects of them were also shown as 2.3 mg/ml for 3-HPAA and 2.1 mg/ml for 4-HBA. The morphological changes of bacteria were determined after phenolic acid exposure via SEM. The LC-ESI-MS/MS technique was used to show changes in the protein profile of bacteria arose from antimicrobial effects. Both phenolic exposures resulted in various protein changes especially in membrane-related proteins as well as ribosome and protein synthesis related-proteins. In addition, they caused serious oxidative stress depending on the protein profile changes related to redox proteins. Alginate-chitosan nanoparticles resulted increased antimicrobial effects of the phenolics which were produced, characterized and tested on various pathogenic bacteria via agar diffusion and spectrophotometric measurements. Hence, free or encapsulated forms of phenolic acids were demonstrated as effective antimicrobial agents and based on proteomic results, the effect of phenolic acids may be multi targeted.

Bacterial infections

Drug resistance

Food contamination & poisoning

Molecular biology

Özdemir, Özgün Öykü

Soyer, Ferda

p

[Thesis]

276903

a

Y