عنوان

Triticum aestivum L. (buğday)'Da gümüş nanopartikülleri Ile oluşturulmuş programlı hücre ölümünün Belirlenmesi

TLpq2460782191

tur

Triticum aestivum L. (buğday)'Da gümüş nanopartikülleri Ile oluşturulmuş programlı hücre ölümünün Belirlenmesi

[Thesis]

Yanık, Fatma

Vardar, Filiz

Marmara Universitesi (Turkey)

2019

214

Ph.D.

Marmara Universitesi (Turkey)

2019

Silver nanoparticles (Ag NPs) have been used in many consumer products due to their antimicrobial and antifungal properties. As a result of widespread usage of silver nanoparticles, it is expected to accumulate in air, water and soil environment. The aim of this study is to determine programmed cell death in wheat roots which were exposed to different concentrations (0.5, 1, 5, 10 and 20 ppm) of 10 nm Ag NPs for 15 d. It was observed that different concentrations of Ag NPs caused root length inhibition, callose deposition and lignin accumulation, but no significant change was observed in shoot length. Cytological and anatomical analyses indicated that Ag NPs lead to accumulation of Ag+ ion, decline in mitotic index, increase in chromosomal anomaly, disruption of nucleus morphology, TUNEL positive reactions and deformation of root anatomy. Based on biochemical analyses, Ag NPs induced alteration of total chlorophyll content and increment of carotenoid and anthocyanin contents in leaves. Ag NPs induced increment of H2O2 content, lipid peroxidation, caspase-1 like activity and alteration of SOD, CAT, GPOX, APOX, GR activities and proline content in roots. Molecular analyses revealed Ag NPs DNA fragmentation, decrease in mitochondrial inner membrane potential, cytochrome c release from mitochondria to cytosol and up-regulation of TaVPE4. 20 metacaspases protein identified by bioinformatic analysis was characterized. It was determined the expression of TaMC1 and TaMC4 metacaspase gene increased during Ag NPs exposure. As a result, it was determined that different concentrations (0.5, 1, 5, 10 and 20 ppm) of Ag NPs induced programmed cell death in wheat roots.

Agricultural engineering

Vardar, Filiz

Yanık, Fatma

p

[Thesis]

276903

a

Y