عنوان

Aspergillus:

TLpq2364201805

انگلیسی

Aspergillus:

[Thesis]

Dávila González, María M.

Interactions among Biofilms, Oxygen, Antifungal Drugs and Melanin

Bayman, Paul

University of Puerto Rico, Rio Piedras (Puerto Rico)

2019

101

M.S.

University of Puerto Rico, Rio Piedras (Puerto Rico)

2019

Aspergillus fungi opportunistic pathogenesis often involves the formation of biofilms and adaptation to low oxygen environments to survive in the human body. However, interactions between biofilms and low oxygen are not well understood. In the first chapter, we studied biofilm formation by A. fumigatus, A. niger, A. flavus, and A. terreus in microaerophilic conditions. All species formed biofilms in microaerophilic conditions. In the second chapter we compared resistance to Amphotericin B in different conditions. A. flavus was more resistant to AMB than A. fumigatus in microaerophilic conditions. In the third chapter, we studied the roles of melanin in biofilm formation and virulence. We evaluate biofilms formation of A. fumigatus mutants deficient in pyomelanin (hppd) and DHN-melanin (alb1). Melanin was not crucial for biofilm formation. Also, the alb1 mutant was more virulent in Drosophila melanogaster than several wild type strains. These data support why aspergillosis infections are challenging to eradicate.

Biology

Microbiology

Bayman, Paul

Dávila González, María M.

p

[Thesis]

276903

a

Y