Improving Knowledge of Microbial Dynamics on Building Materials under High Moisture Conditions
[Thesis]
Zhao, Dan
Stephens, Brent Robert
Illinois Institute of Technology
2020
210 p.
Ph.D.
Illinois Institute of Technology
2020
Most buildings experience some kind of high moisture event(s) throughout their life cycles, often resulting from water leaks or migration of water vapor through the enclosure. Dampness and moisture in buildings leads to fungal growth and is associated with adverse human health outcomes. Although the dynamics of fungal growth on buildings materials has been investigated for decades, few studies have integrated modern chemical or microbiological analytical methods (e.g., DNA sequencing, qPCR, etc.) to understand microbial dynamics on materials held at high humidity conditions. Moreover, most mold growth prediction models remain relatively simplistic and rely solely on empirical data for visible mold growth. To bridge some of these gaps, this research aims to improve understanding of microbial growth and community dynamics on building materials under high moisture conditions and to improve our ability to predict microbial growth and community dynamics under a variety of conditions. Five distinct but overlapping research objectives are used to achieve these goals, including: (1) evaluating the growth of microorganisms on wetted building materials and identifying relationships between specific microbial taxa, metabolites, and environmental variables; (2) identifying inherent material chemistry drivers of fungal growth susceptibility and their relation to microbial community structure; (3) exploring how fluctuating moisture exposures impact bacterial and fungal growth and dynamics on building materials; (4) investigating microbial interactions using isolated communities on a single material; and (5) evaluating and improving existing mathematical mold growth models.