Foreword / Anne-Marie Catesson -- 1. Introduction / Nigel Chaffey -- 2. An Introduction to the Problems of Working with Trees / Nigel Chaffey -- 3. Wood Microscopical Techniques / Nigel Chaffey -- 4. Conventional (Chemical-fixation) Transmission Electron Microscopy and Cytochemistry of Angiosperm Trees / Nigel Chaffey -- 5. Chemical and Cryo-fixation for Transmission Electron Microscopy of Gymnosperm Cambial Cells / Kim H. Rensing -- 6. Deep-etching Electron Microscopy and 3-dimensional Cell Wall Architecture / Takao Itoh -- 7. Secondary Ion Mass Spectrometry Microscopy: Application to the Analysis of Woody Samples / Marie-Laure Follet-Gueye -- 8. Immunolocalisation of the Cytoskeleton in the Secondary Vascular System of Angiosperm Trees and its Visualisation Using Epifluorescene Microscopy / Nigel Chaffey -- 9. Immunolocalisation and Visualisation of the Cytoskeleton in Gymnosperms using Confocal Laser Scanning Microscopy / Ryo Funada -- 10. Cell Walls of Woody Plants: Autoradiography and Ultraviolet Microscopy / Keiji Takabe -- 11. Cell Walls of Woody Tissues: Cytochemical, Biochemical and Molecular Analysis of Pectins and Pectin Methylesterases / Fabienne Micheli, Fabienne E. Ermel and Marianne Bordenave / [et al.] -- 12. Immunolocalisation of Enzymes of Lignification / Jozef Samaj and Alain Michel Boudet -- 13. Sampling of Cambial Region Tissues for High Resolution Analysis / Claes Uggla and Bjorn Sundberg -- 14. Biochemistry and Quantitative Histochemistry of Wood / Elisabeth Magel -- 15. Protein Analysis in Perennial Tissues / Tannis Beardmore, Suzanne Wetzel and Carrie-Ann Whittle -- 16. The Use of GUS Histochemistry to Visualise Lignification Gene Expression In Situ during Wood Formation / Simon Hawkins, Gilles Pilate and Eric Duverger / [et al.] -- 17. High Resolution In Situ Hybridisation in Woody Tissues / Sharon Regan and Bjorn Sundberg -- 18. Random Amplification of Polymorphic DNA and Reverse Transcription Polymerase Chain Reaction of RNA in Studies of Sapwood and Heartwood / Elisabeth Magel, Siegfried Hauch and Luigi De Filippis
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Trees are a major component of the biosphere and have played an important part in the world's history and culture. With the modern challenges of global warming and dwindling fossil fuel reserves, trees, and in particular their wood, can provide solutions. Unfortunately, too little is known about the biology of these plants, due largely to a lack of appropriate techniques. In recognition of this, this book presents a variety of detailed techniques and protocols for the study of the cell and molecular biology of wood formation in trees. The techniques reflect a hierarchical approach to the study of the developmental biology of wood formation: anatomical, biochemical and molecular-genetic. Trees are a tremendous but vastly under-appreciated natural resource. In an age where the natural product is so often modified to suit modern tastes and industrial processes, it is essential to understand how the natural product is made. The techniques in this book provide that essential information about the process of wood formation in trees.