عنوان

Protein localization by fluorescent microscopy :

0199637407

0199637415

9780199637409

9780199637416

b792418

Protein localization by fluorescent microscopy :

[Book]

a practical approach /

edited by Victoria J. Allan.

New York :

Oxford University Press,

2000.

xxiv, 231 pages :

illustrations (some color) ;

24 cm.

The practical approach series ;

218

Includes bibliographical references and index.

1. Basic immunofluorescence / Victoria J. Allan -- 2. Immunofluorescent labelling of sections / Alison J. North and J. Victor Small -- 3. Simultaneous in situ detection of DNA and proteins / Klaus Ersfeld and Elisa M. Stone -- 4. Instruments for fluorescence imaging / W.B. Amos -- 5. Fluorescence microscopy of living vertebrate cells / Rainer Pepperkok and David Shima -- 6. Visualizing fluorescence in Drosophila -- optimal detection in thick specimens / Ilan Davis -- 7. Green fluorescent protein in plants / Chris Hawes, Petra Boevink and Ian Moore -- 8. Fluorescence microscopy in yeast / Iain M. Hagan and Kathryn R. Ayscough -- App. A1. Excitation and emission maxima of commonly used fluorophores -- App. A2. Commercial sources of antibodies -- App. A3. Optical units -- App. A4. List of Suppliers.

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"An ever-increasing number of genes that are being sequenced have no known function. Determining the location of such gene products within the cell, either by the use of antibodies or by the production of chimeras with green fluorescent protein, is a vital step towards understanding what they do. This is one major reason why fluorescence microscopy is enjoying a revival. This no-nonsense guide provides detailed, practical advice on all aspects of the subject: from choosing the right equipment, to interpreting results. It balances the advantages of a wide range of techniques - including live cell work - against the potential pitfalls, offering invaluable 'tricks of the trade' along the way. Protein Localization by Fluorescence Microscopy: A Practical Approach has something to offer all microscopists, giving a solid grounding for the novice whilst also extending the range of the experienced user."--Jacket.

Fluorescence microscopy.

Green fluorescent protein.

Immunofluorescence.

Proteins-- Analysis.

Luminescent Proteins.

Microscopy, Fluorescence.

Sequence Analysis, Protein.

Fluorescence microscopy.

Green fluorescent protein.

Immunofluorescence.

Immunofluorescence.

Microscopie de fluorescence.

Protéine fluorescente verte.

Protéines-- Analyse.

Proteins-- Analysis.

X400

Allan, Victoria J.

20201207054110.0

[Book]

Y