Front Cover; Methods in Cell Biology, Volume XX; Copyright Page; Contents; List of Contributors; Preface; Chapter 1. Measuring Spontaneous Mutation Rates in Yeast; I. Introduction; II. Measuring the Spontaneous Mutation Rate in Mitotic Cells by Limiting a Required Nutrient; III. Measuring the Spontaneous Mutation Rate in Mitotic Cells Grown in Complete Medium; IV. Measuring the Spontaneous Mutation Rate during Meiosis; V. Conclusion; Appendix: Biometrical Aspects of Measuring Mutation Rates; References; Chapter 2. Manganese Mutagenesis in Yeast; I. Introduction
Chapter 6. Method for Yeast Protein Synthesis in a Cell-Free SysternI. Introduction; II. Early Yeast Cell-Free Systems; III. The S-30 Extract; IV. Assay of Protein Synthesis; V. Prospects; References; Chapeter 7. Preparation of Protoplasts of Schizosaccharomyces pombe; I. Introduction; II. Preparation of Protoplasts; III. Concluding Remarks; References; Chapter 8. Bulk Isolation of Yeast Mitochondria; I. Introduction; II. Materials and Methods; III.Breakage of Yeast Cells; IV.Isolation of Mitochondria; V.Properties of Isolated Mitochondria; VI.Discussion; References
Chapter 9. Isolation, Regeneration, and Fusion of Protoplasts of PhycomycesI. Introduction; II. Material; III. Preparation of Cells; IV.Protoplast Isolation; V.Reversion of Protoplasts; VI.Protoplast Fusion; VII.Discussion; References; Chapter 10.The Neurospora Plasma Membrane: A New Experimental System for Investigating Eukaryote Surface Membrane Structure and Function; I. Introduction; II.Isolation of Neurospora Plasma Membranes; III.Properties of Isolated Neurospora Plasma Membranes; IV.Conclusion; References; Chapter 11. Staining Fungal Nuclei with Mithramycin; I.Introduction
I. Preparation of RibosomesII. Preparation of Ribosomal Proteins; III. Analysis of Ribosomal Proteins; IV. Conclusion; References; Note Added in Proof; Chapter 5. Isolation, Characterization, and Translation of mRNA from Yeast; I. Media, Cells, and Spheroplasts; II. Isolation of Polysomes, Single Ribosomes, and Ribosomal Subunits; III. Detection of mRNA in Polysomes and Subunits; IV. Preparation of RNA; V. Analysis of RNA on Polyacrylamide Gels; VI. Isolation of Poly-A-Containing RNA; VII. Analysis of 5'-Termini of mRNA; VIII. Translation of Yeast mRNA; References
II. Induction of Mitochondrial MutationsIII. Induction of Nuclear Mutations; IV. The Uptake of Manganese and Its General Effects on Yeast Cells; V. Other Mutagens Inducing Mutations Nonlethal for rho Factor; References; Chapter 3. Yeast Cell Selection and Synchrony: Density Gradients and Mating Factor Block; I. Introduction; II. lsopycnic Density Gradients; III. Isolation of Cells Labeled at Specific Periods of the Cell Cycle; IV. a- and a-Factor Selection and Synchronization of Haploid Yeast Cells; V. Discussion; References; Chapter 4. The Ribosomal Proteins of Saccharomyces cerevisiae