عنوان

Biological aging :

158829658X

1597453617

9781588296580

9781597453615

b788575

Biological aging :

[Book]

methods and protocols /

edited by Trygve O. Tollefsbol.

Totowa, N.J. :

Humana Press,

©2007.

1 online resource (xv, 414 pages) :

illustrations (some color)

Methods in molecular biology ;

371

Includes bibliographical references and index.

Techniques for analysis of biological aging / Trygve O. Tollefsbol -- Aging cell culture: methods and observations / Sharla M.O. Phipps [and others] -- Methods to detect biomarkers of cellular senescence: the senescence-associated [beta]-galactosidase / Koji Itahana, Judith Campisi, and Goberdhan P. Dimri -- Methods of cell sorting of young and senescent cells / João F. Passos and Thomas von Zglinicki -- Telomere length analysis / Andrés Canela, Peter Klatt, and María A. Blasco -- A method to detect DNA methyltransferase I gene transcription in vitro in aging systems / Joel B. Berletch, Lucy G. Andrews, and Trygve O. Tollefsbol -- A method to study the expression of DNA methyltransferase in aging systems in vitro / Joel B. Berletch [and others] -- The chronological life span of Saccharomyces cerevisiae / Paola Fabrizio and Valter D. Longo -- Caloric restriction and life span determination of yeast cells / Oliver Medvedik and David A. Sinclair -- Methods of nutrigenomics and longevity studies in drosophila: effects of diets high in sucrose, palmitic acid, soy, or beef / Jiatao Ye [and others] -- Caloric restriction mimetics: a novel approach for biogerontology / Mark A. Lane, George S. Roth, and Donald K. Ingram -- Extension of cell life span using exogenous telomerase / Mo K. Kang and No-Hee Park -- Inducing cellular senescence using defined genetic elements / Hiroshi Nakagawa and Oliver G. Opitz -- Methods of induction of cellular senescence using oxidative stress / Jian-Hua Chen, Susan E. Ozanne, and C. Nicholas Hales -- Nuclear transfer methods to study aging / Lin Liu and David L. Keefe -- Use of ribozymes in cellular aging research / Custer C. Deocaris, Sunil C. Kaul, and Renu Wadhwa -- Methods of evaluating the effect of pharmacological drugs on aging and life span in mice / Vladimir N. Anisimov, Irina G. Popovich, and Mark A. Zabezhinski -- Identifying genes that extend life span using a high-throughput screening system / Cuiying Chen and Roland Contreras -- Application of DNA microarray technology to gerontological studies / Sergey V. Anisimov -- Detection and analysis of somatic mutations at a lacZ reporter locus in higher oranisms: application to Mus musculus and Drosophila melanogaster / Ana Maria Garcia [and others] -- Subtractive hybridization techniques to study cellular senescence / Michal Bassani Sternberg and Shimon Gepstein -- Mapping genetic polymorphisms affecting natural variation in drosophila longevity / Maria De Luca and Jeff Leips -- Quantitative trait locus (QTL) mapping in aging systems / Hui-Chen Hsu [and others] -- 2D gel proteomics: an appraoch to study age-related differences in protein abundance or isoform complexity in biological samples / Helen Kim [and others] -- Metabolomics in the study of aging and caloric restriction / Bruce S. Kristal [and others].

0

Biological Aging: Methods and Protocols investigates the various processes that are affected by the age of an organism. Several new tools for the analysis of biological aging have been introduced recently, and this volume provides methods and protocols for these new techniques in addition to its coverage of established procedures. The editors have carefully selected only those topics that are considered mainstays of the field or are showing promise in revolutionizing this relatively new science. The three main areas of focus in this cutting-edge compendium of biological aging research are: methods that are basic to understanding the fundamental mechanisms of cellular aging; techniques used to intervene in the aging process; and approaches to analyzing the many molecular processes of biological aging. Researchers seeking new technology and techniques will find this volume of tremendous benefit as they move towards new directions in the exciting and expanding field of biological aging.

MIL

97160

Biological aging.

9781588296580

Aging-- Physiological aspects, Laboratory manuals.

Longevity, Laboratory manuals.

Aging-- physiology.

Biomarkers.

Cellular Senescence-- physiology.

Longévité, Manuels de laboratoire.

Vieillissement-- Aspect physiologique, Manuels de laboratoire.

Aging-- Physiological aspects.

FAMILY & RELATIONSHIPS-- Aging.

Longevity.

MEDICAL-- Nursing-- Gerontology.

FAM-- 005000

MED-- 058060

MJX

Tollefsbol, Trygve O.

20201207020633.0

pn

[Book]

Y