عنوان

Handbook of cryo-preparation methods for electron microscopy /

0849372275

9780849372278

b787707

Handbook of cryo-preparation methods for electron microscopy /

[Book]

edited by Annie Cavalier, Danièle Spehner, Bruno M. Humbel.

Boca Raton :

CRC Press,

©2009.

xix, 682 pages :

illustrations ;

26 cm.

Methods in visualization

Includes bibliographical references and index.

Slam-freezing, metal-mirror freezing / Danièle Spehner and Ludwig Edelmann -- Plunge-freezing (Holey carbon method) / Sacha De Carlo -- Controlled vitrification / Peter M. Frederik, Felix de Haas, and Marc M.H. Storms -- BAL-TEC HPM 010 high-pressure freezing machine / Andres Kaech -- High-pressure freezing Leica EMPACT / Dimitri Vanhecke and Daniel Studer -- Frozen-hydrated macromolecules for structural analysis / Corinne Crucifix, Gabor Papai and Patrick Schultz -- Two-dimensional crystals / Patrick Bron and Janet Vonck -- Cryo-negative staining / Sacha De Carlo -- Vitrification of dynamic microtubules / Isabelle Arnal, Marine Malleter, and Denis Chrétien -- CEMOVIS : cryo-electron microscopy of vitreous sections / Jacques Dubochet [and others] -- Cryo-electron tomography / Andrew Leis, Manuela Gruska, and Wolfgang Baumeister -- Freeze-substitution / Bruno M. Humbel -- Cryo-fixation, freeze-substitution, rehydration and Tokuyasu cryo-sectioning / York-Dieter Stierhof [and others] -- Freeze-drying and embedding of biological material / Ludwig Edelmann -- The shadow of hydrated biological specimens / Daniel Thomas -- Cryo-fracture of self assembled systems in organic solvent / Marc Schmutz and Philippe J. Mésini -- Progressive lowering of temperature for immunolabeling and in situ hybridization / Annie Cavalier and Danièle Spehner -- Cryo-sectioning according to Tokuyasu / Bruno M. Humbel and York-Dieter Stierhof -- Cryo-preparation procedures for elemental imaging by SIMS and EFTEM / Christian Bordat and Jean-luc Guerquin-Kern -- Correlative light and electron microscopy / Heinz Schwarz and Bruno M. Humbel -- SDS-digested freeze-fracture replica labeling (SDS-FRL) / Yugo Fukazawa [and others] -- Immunolabeling of ultrathin sections with enlarged 1 nm gold or QDots / York-Dieter Stierhof -- 3-D electron tomography of cells and organelles / Willie J.C. Geerts, Bruno M. Humbel and Arie J. Verkleij -- Final considerations / Hellmuth Sitte.

0

"Collecting the most sophisticated methods used today, Handbook of Cryo-Preparation Methods for Electron Microscopy calls on internationally renowned scientists, some the very inventors of the methods they describe, to share their knowledge and recipes. Taking care to explain the history behind the techniques and demonstrate their use, this book presents theory, principles, and protocols supplemented by comments, tips and advice and more than 400 images and illustrations from the contributors' own laboratories. With clear, step-by-step recipes and lists of tools, ingredients, and suppliers, this handbook enables the ready application of the latest cryo-methods to current research and fosters future developments in an accessible, didactic, and pedagogic fashion."--BOOK JACKET.

Cryobiology-- Methodology.

Electron microscopy-- Methodology.

Cryopreservation-- methods.

Microscopy, Electron-- methods.

Cryobiologie-- Méthodologie.

Microscopie électronique-- Méthodologie.

Electron microscopy-- Methodology.

Elektronenmikroskopie

Kryobiologie

Cavalier, Annie.

Humbel, Bruno M.

Spehner, Daniele.

20201207012319.0

[Book]

Y