Nucleases: an overview / A. Fred Weir -- Deoxyribonuclease I (EC 3.1.21.1) and II (EC 3.1.22.1) / A. Fred Weir -- DNA polymerases (EC 2.7.7.7) / Martin J. Maunders -- Taq polymerase (EC 2.7.7.7): with particular emphasis on its use in PCR protocols / Axel Landgraf and Heiner Wolfes -- Eukaryotic nuclear RNA polymerases (EC 2.7.7.6) / Deborah A. Cook and Robert J. Slater -- Reverse transcriptase (EC 2.7.7.49): the use of cloned moloney murine leukemia virus reverse transcriptase to synthesize DNA from RNA / Gary F. Gerard and James M. D'Alessio -- Terminal deoxyribonucleotidyl transferase (EC 2.7.7.31) / Frank Grosse and Andreas Manns -- Restriction enzymes / Alfred Pingoud, Jurgen Alves and Robert Geiger -- DNA methyltransferases (EC 2.1.1.72 and EC 2.1.1.73) / David P. Hornby -- DNA and RNA ligases (EC 6.5.1.1, EC 6.5.1.2 and EC 6.5.1.3) / Martin J. Maunders -- BAL 31 nucleases (EC 3.1.11) / Horace B. Gray, Jr. and Tao Lu -- Mung-bean nuclease 1 (EC 3.1.11) / Andrew J. Sharp and Robert J. Slater -- RNase A (EC 3.1.27.5) / Michael M. Burrell -- Pronase (EC 3.4.24.4) / Patricia J. Sweeney and John M. Walker -- Proteolytic enzymes for peptide production / Patricia J. Sweeney and John M. Walker -- Proteinase K (EC 3.4.21.14) / Patricia J. Sweeney and John M. Walker -- Carboxypeptidase Y (EC 3.4.16.1) / Julia S. Winder and John M. Walker -- Aminopeptidases: aminopeptidase M (EC 3.4.11.2), pyroglutamate aminopeptidase (EC 3.4.19.3) and prolidase (EC 3.4.13.9) / Patricia J. Sweeney and John M. Walker -- Alkaline phosphatase (EC 3.1.3.1) / Martin J. Maunders -- Polynucleotide kinase (EC 2.7.1.78) / Martin J. Maunders.