Measurement of bioaccessibility of organic pollutants in soil / Laura McAllister and Kirk T. Semple -- Heavy metal bioavailability and bioaccessibility in soil / John Richard Dean -- Addressing PCR biases in environmental microbiology studies / Rita Sipos [and others] -- MPN- and real-time-based PCR mehods for the quantification of alkane monooxygenase homologous genes (alkB) in environmental samples / Alfredo Pérez-de-Mora, Stephan Schulz, and Michael Schloter -- Application of fingerprinting molecular methods in bioremediation studies / Dimitrios G. Karpouzas and Brajesh K. Singh -- Using terminal restriction fragment length polymorphism (T-RFLP) analysis to assess microbial community structure in compost systems / Soni M. Tiquia -- Combination fluorescence in situ hybridization with staining techniques for cell viability and accumulation of PHA and polyP in microorganisms in complex microbial systems / Jeppe Lund Nielsen, Caroline Kragelund, and Per Halkjær Nielsen -- Ecophysiological analysis of microorganisms in complex microbial systems by combination of fluorescence in situ hybridization with extracellular staining techniques / Jeppe Lund Nielsen, Caroline Kragelund, and Per Halkjær Nielsen -- Applications of DNA-stable isotope probing in bioremediation studies / Yin Chen, Jyotsna Vohra, and J. Colin Murrell --Two-dimensional gel electrophoresis : discovering biomolecules for environmental bioremediation / Om V. Singh and Anuj K. Chandel -- The application of molecular techniques to the study of wastewater treatment systems / Irena Wojnowska-Baryła, Agnieszka Cydzik-Kwiatkowska, and Magdalena Zielińska -- Plasmid-mediated bioaugmentation of wastewater microbial communities in a laboratory-scale bioreactor / Stephan Bathe and Martina Hausner -- The role of decision support for bioremediation strategies, exemplified by hydrocarbons for in site and ex situ procedures / Elizabeth E. Diplock [and others] -- Monitored natural attenuation / Kirsten S. Jorgensen, Jani M. Salminen, and Katarina Björklöf -- Application of bioassays for the ecotoxicity assessment of contaminated soils / Maria D. Fernández, Mar Babín, and José V. Tarazona -- Community-level physiological profiling / Kela P. Weber and Raymond L. Legge.
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Since its first systematic application during the 1970s, bioremediation, or the exploitation of a biological system's degradative potential to combat toxic pollutants such as heavy metals, polyaromatic hydrocarbons (PAH), cyanides, and radioactive material, has proven itself over time, and the many advances in molecular techniques have only amplified its utility. In Bioremediation: Methods and Protocols, experts in the field explore imaginative and ambitious multidisciplinary techniques that will enable more predictable removal of pollutants from a variety of environments. The easy-to-follow volume addresses some of the broader issues such as the effect of the environment in determining the availability and fate of organic and inorganic compounds and how choices around the most appropriate bioremediation process can be arrived at, as well as detailed complementary techniques that support the effective deployment and monitoring of a bioremediation approach. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bioremediation: Methods and Protocols offers researchers a series of invaluable techniques certain to enhance their work with environmental contamination.
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