The retroviral life cycle and the molecular construction of retrovirus vectors / Richard Vile -- Construction of retro viral packaging cell lines / Olivier Danos -- Making high titer retroviral producer cells / Stephen J. Russell -- Methods of titrating nonselectable recombinant retroviruses / Colin D. Porter -- Selectable markers for eukaryotic cells / Richard Vile -- Virus genome detection by the polymerase chain reaction (PCR) / Julian G. Hoad, Thomas F. Schulz -- Syncytial assays / Myra O. McClure -- Neutralization of virus / Jane McKeating -- Vesicular stomatitis virus pseudotypes of retroviruses / Paul R. Clapham -- Cell lineage studies using retroviruses / Jack Price -- Retroviral vectors as insertional mutagens / Joop Gäken, Farzin Farzaneh -- Assessment of compounds for anti-HIV activity / Clive Patience, John Moore, Mark Boyd -- Analysis of protein factors that interact with adenovirus early promoters / Helen C. Hurst -- COS cell expression / John F. Hancock -- Papillomaviruses and assays for transforming genes / Karen H. Vousden -- Herpes simplex virus life cycle and the design of viral vectors / David S. Latchman -- Growth of herpes simplex virus and purification of viral DNA / David S. Latchman, Lynn M. Kemp -- Vectors based on Epstein-Barr virus / Paul M. Brickell, Mukesh Patel -- Immortalization of human B-lymphocytes by Epstein-Barr virus / Paul M. Brickell -- Vaccinia virus as an expression vector / Antonio Talavera, Javier M. Rodriguez -- Isolation and handling of recombinant vaccinia viruses / Antonio Talavera, Javier M. Rodriguez -- Design and construction of poliovirus epitope expression vectors / Karen L. Burke, Jeffrey W. Almond, David J. Evans -- Growth and characterization of poliovirus antigen chimeras / David J. Evans, Philip D. Minor -- Expression vectors for the construction of hybrid Ty-VLPs / Sally E. Adams, S. Mark, H. Richardson, Susan M. Kingsman, Alan J. Kingsman -- Production and purification of hybrid Ty-VLPs / Nigel R. Burns, Jacqueline E. Gilmour, Susan M. Kingsman, Alan J. Kingsman, Sally E. Adams -- Baculovirus expression vectors. Choice of expression vector / Vincent C. Emery -- Baculovirus expression vectors Generating a recombinant baculovirus / Vincent C. Emery -- Baculovirus expression vectors. Scale-up and downstream / Vincent C. Emery.