Chapter 9. Delivery of Macromolecules into Cells Expressing a Viral Membrane Fusion Protein.
Front Cover -- Methods in Cell Biology, Volume 31 -- Copyright Page -- Contents -- Contributors -- Preface -- PART I: GAINING ACCESS TO THE CYTOPLASM -- Chapter 1. Lysed Chromatophores: A Model System for the Study of Bidirectional Organelle Transport -- I. Introduction -- II. Methods to Obtain, Lyse, and Reactivate Chromatophores -- III. Mechanisms of Organelle Transport in Chromatophores -- IV. Summary -- References -- Chapter 2. Digitonin Permeabilization Procedures for the Study of Endosome Acidification and Function -- I. Introduction.
II. Preparation of Transport-Competent Membranes -- III. Reconstitution of Protein Transport -- IV. Future Prospects -- References -- Chapter 8. Reconstitution of Transport from the ER to the Golgi Complex in Yeast Using Microsomes and Permeabilized Yeast Cells -- I. Introduction -- II. In Vitro Translation of Prepro--factor in a Yeast Lysate -- III. In Vitro Translocation into the ER Lumen of PYC or Microsomes -- IV. In Vitro Transport of Pro--factor from the ER to the Golgi Complex -- V. Summary -- References.
II. Prepartion of Mannose-BSA for Endosome Acidification Studies -- III. Permeabilization of Cells -- IV. Endosome Acidification -- V. Summary and Outlook -- References -- Chapter 3. ATP Permeabilization of the Plasma Membrane -- I. Introduction -- II. Plasma Membrane Receptors for Adenine Nucleotides -- III. ATP Permeabilization -- IV. Recovery of Intracellular Homeostasis following ATP Permeabilization -- V. Assessment of Permeabilization -- VI. ATP-Resistant Cells -- VII. Uses of ATP Permeabilization -- VIII. Relative Merits of ATP as a Means of Membrane Permeabilization.
III. Preparation of Semiintact Cells from Adherent Cells -- IV. Preparation of Semiintact Cells from Suspension Cells -- V. Incubation Conditions to Achieve Transport between the ER and Golgi in Vitro -- VI. Discussion -- References -- Chapter 6. Perforated Cells for Studying Intracellular Membrane transport -- I. Introduction -- II. Generation and Characterization of Perforated Cells -- III. Reconstitution of Intracellular Membrane transport -- IV. Discussion -- References -- Chapter 7. Reconstitution of Protein Transport Using Broken Yeast Spheroplasts -- I. Introduction.
IX. Mechanism of ATP-Induced Permeabilization -- X. Conclusion -- References -- Chapter 4. Poration by -Toxin and Streptolysin 0: An Approach to Analyze Intracellular Processes -- I. Introduction -- II. Purification and Analysis of Pore-Forming Toxins -- III. Application for Cell Poration -- IV. Pore-Forming Toxins as Tools in the Study of Intracellular Processes -- V. Concluding Remarks -- References -- Chapter 5. Preparation of Semiintact Chinese Hamster Ovary Cells for Reconstitution of Endoplasmic Reticulum-to-Golgi transport in a Cell-Free System -- I. Introduction -- II. Growth of Cells.
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METHODS IN CELL BIOLOGY, VOLUME 31: VESICULAR TRANSPORT, PART A.
METHODS IN CELL BIOLOGY, VOLUME 31: VESICULAR TRANSPORT, PART A.