Front Cover; Confocal Microscopy; Copyright Page; Table of Contents; Contributors to Volume307; Preface; Volumes in Series; Section I: Theory and Practical Considerations; Section II: General Techniques; Section III: Measurement of Subcellular Relations and Volume Determinations; Section IV: Imaging of Processes; Section V: Imaging of Ions; Section VI: Imaging of Specialized Tissues; Section VII: Imaging Viruses and Fungi; Author Index; Subject Index.
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This volume supplements Volumes 63, 64, 87, and 249 of Methods in Enzymology. These volumes provide a basic source for the quantitative interpretation of enzyme rate data and the analysis of enzyme catalysis. Among the major topics covered are Engergetic Coupling in Enzymatic Reactions, Intermediates and Complexes in Catalysis, Detection and Properties of Low Barrier Hydrogen Bonds, Transition State Determination, and Inhibitors. The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemi.