عنوان

Carbohydrate-based interactions at the molecular and the cellular level /

3030085171

3319777068

3319777076

9783030085179

9783319777061

9783319777078

331977705X

9783319777054

Carbohydrate-based interactions at the molecular and the cellular level /

[Book]

Kieran L. Hudson.

Cham :

Springer,

2018.

1 online resource

Springer theses

Intro; Supervisors' Foreword; Abstract; Parts of this thesis have been published in the following journal articles:; Acknowledgements; Contents; Abbreviations and Acronyms; List of Figures; List of Tables; List of Schemes; 1 Introduction; 1.1 Carbohydrates in Nature; 1.1.1 Carbohydrate Structure; 1.2 Protein-Carbohydrate Interactions; 1.2.1 The Roles and Importance of Protein-Carbohydrate Interactions in Nature; 1.2.2 Analysis of Protein-Carbohydrate Interactions; 1.2.3 The Molecular Basis of Protein-Carbohydrate Interactions; 1.2.4 Carbohydrate-Aromatic Interactions.

1.3 Scope of the Thesis Part I: Analysis of Protein-Carbohydrate Interactions in Protein Structures1.4 Tissue Engineering; 1.4.1 Tissue Engineering Scaffolds; 1.4.2 Hydrogels Derived from -Helical Peptides; 1.4.3 Functionalisation of Tissue Engineering Scaffolds; 1.4.4 Carbohydrates in Tissue Engineering; 1.5 Carbohydrate Synthesis; 1.5.1 Chemical Synthesis of Carbohydrates; 1.5.2 Enzymatic Synthesis of Carbohydrates; 1.6 Scope of the Thesis Part II: Tissue Engineering Scaffolds Functionalised with Carbohydrates; References.

3.5 Studies of Carbohydrate-Aromatic Interactions in the Solution Phase3.5.1 Influence of C-H Electronics on CH- Interactions; 3.5.2 Influence of Aromatic Electronics on CH- Interactions; References; Part II Carbohydrate-Modified Materials for Tissue Engineering; 4 The Modifiable Scaffold for Tissue Engineering; 4.1 The Structural Basis of hSAFs; 4.2 Peptide Synthesis and Hydrogel Preparation; 4.3 Characterisation and Properties of hSAFs; 4.4 Decoration of hSAFs with Functional Motifs; 4.4.1 Properties of the Modifiable and Modified Scaffold.

Part I Analysing Protein#x96;Carbohydrate Interactions at the Molecular Level2 Protein-Carbohydrate Interactions in Protein X-Ray Crystal Structures; 2.1 Identifying Carbohydrates in Protein Structures; 2.1.1 Using GlyVicinity to Determine Carbohydrate Identity; 2.1.2 Parsing the GlyVicinity Output; 2.1.3 Generating the Data Set of Carbohydrates with Proximal Amino Acids; 2.2 Analysis of the Composition and Distribution of Amino Acids Surrounding Carbohydrates; 2.3 Identifying CH- Interactions; 2.4 Validating the Quality of the Structural Data; 2.5 Comparison of Data for Ligands and Glycans.

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This book offers a clearly written and highly accessible account of two different aspects of carbohydrate chemistry. Carbohydrates are an essential component of life and have many important biological functions, but the details of how carbohydrates interact with other biomolecules to mediate biological signalling remain unclear. Firstly, this thesis details innovative methods to mine protein structural data to uncover new features of carbohydrate-based interactions. It also explains these findings using physical chemistry, specifically CH-pi interactions associated with the properties of the interacting partners. Carbohydrates are also critical for tissue growth and development, yet are underexploited in the materials science that underpins much of regenerative medicine. As such, the second part of this thesis describes a diverse array of techniques ranging from synthetic chemistry and enzymatic synthesis to prepare a wide variety of carbohydrates, and materials chemistry to prepare glycosylated hydrogels, to cell biology to determine the effects on cellular development for tissue engineering applications.

Springer Nature

com.springer.onix.9783319777061

Carbohydrate-based interactions at the molecular and the cellular level.

9783319777054

Molecular dynamics.

Sugar-- Analysis.

Molecular dynamics.

SCIENCE-- Chemistry-- Physical & Theoretical.

Sugar-- Analysis.

PSB

PSBF

SCI-- 013050

Hudson, Kieran L.

20200823112449.0

pn

[Book]

Y