عنوان

Membrane proteins in aqueous solutions :

3319731467

3319731483

9783319731469

9783319731483

3319731467

9783319731469

Membrane proteins in aqueous solutions :

[Book]

from detergents to amphipols /

by Jean-Luc Popot.

Cham, Switzerland :

Springer,

[2018]

1 online resource (xxvii, 708 pages) :

illustrationssome color

Biological and Medical Physics, Biomedical Engineering,

1618-7210

Includes bibliographical references and index.

Membrane proteins and their natural environment -- Extracting membrane proteins from their native environment -- Alternatives to detergents for handling membrane proteins in aqueous solutions -- Chemical structure, synthesis, and physical-chemical properties of amphipols -- Formation and properties of membrane protein/amphipol complexes -- Amphipol-assisted folding of membrane proteins -- Amphipol-assisted cell-free expression of membrane proteins -- Optical spectroscopy of membrane protein/amphipol complexes -- Solution studies of membrane protein/amphipol complexes -- Nuclear magnetic resonance studies of amphipol-trapped membran proteins -- Amphipols and membrane protein crystallization -- The use of amphipols for electron microscopy -- Amphipol-mediated immobilization of membrane proteins and its applications -- The use of amphipols in mass spectrometry -- Biomedical applications -- Final comments.

0

Membrane proteins represent about one third of the proteins encoded in a cell's genome, and, because of their key physiological roles, more than half of drug targets. Detergents are traditionally used to extract proteins from membranes in order to make them amenable to the tools of biochemistry and biophysics. However, detergent-solubilized proteins are generally unstable. This has led to the development of alternative, non-conventional surfactants, such as bicelles, nanodiscs, amphipathic peptides, fluorinated surfactants, and specially designed amphipathic polymers called 'amphipols'. These novel tools, mainly developed over the past 20 years, are revolutionizing handling membrane proteins in vitro for basic and applied research, as well as for such biomedical applications as drug screening or vaccination. This book, written by a specialist of membrane proteins and one of the creators of amphipols, describes the properties and uses of these novel molecules. It opens with general introductions on membrane proteins and their natural environment, detergents, the current status of membrane protein in vitro studies, a broad panorama of non-conventional surfactants and a discussion of their respective advantages and limitations, and the preparation and properties of amphipols and membrane protein/amphipol complexes. Topical chapters cover in vitro folding, cell-free synthesis and stabilization of membrane proteins, and such biophysical and biochemical applications as electron microscopy, Xray diffraction, NMR, optical spectroscopy, mass spectrometry, the whole range of solutions studies, proteomics, and such practical applications as membrane protein immobilization and drug screening and the use of amphipols in vivo for vaccination and drug delivery. Each topical chapter is introduced with a concise, up-to-date overview of how membrane proteins are currently studied using each individual technique, before offering an exhaustive coverage and in-depth discussion of the contribution of amphipols, and concluding with hands-on protocols written by everyday practitioners of each application. In addition to a comprehensive coverage of the properties and uses of non-conventional surfactants, this book therefore also offers a concise, accessible introduction to membrane protein biochemistry and biophysics. It is meant to be used both in basic and applied research laboratories and as a teaching help.

00024965

9783319731469

9783319731469

Biosurfactants.

Detergents.

Membrane proteins.

Pharmaceutical chemistry.

Pharmaceutical technology.

Polymers.

Membrane Proteins.

Biochemistry.

Biophysics.

Chemistry, Physical and theoretical.

Pharmaceutical chemistry.

Pharmaceutical technology.

Pharmaceutical technology.

Physical chemistry.

Physics.

Polymer chemistry.

Polymers.

Proteins.

Proteins.

SCIENCE-- Life Sciences-- Biochemistry.

PHVD

PHVN

SCI-- 007000

Popot, Jean-Luc

20200823104039.0

pn

[Book]

Y