عنوان
پدید آورنده
موضوع
رده
کتابخانه
محل استقرار
TLets484291
Fluorigenic enzyme assays using long-wavelength substrates
[Thesis]
Sarpara, Ghulam Hussain
Loughborough University
2000
Thesis (Ph.D.)
2000
Investigations into the use of long-wavelength fluorigenic substrates, which are non- or weakly fluorescent but are converted by an appropriate enzyme to a highly fluorescent product, have been carried out in order to determine several different enzymes and their inhibitors. Certain nonfluorescent compounds based on long-wavelength xanthenes and oxazines dyes were synthesised. Amongst these, naphthofluorescein diphosphate and naphthofluorescein mono-phosphate has been demonstrated to be new fluorigenic substrates for alkaline phosphatase. The hydrolysis product of these substrates can be used for trace analyses using diode laser based fluorescence detection.
Organic chemistry
Sarpara, Ghulam Hussain
Loughborough University
مطالعه متن کتاب p
[Thesis]
276903
a
Y
