عنوان

Methods for Rice Phenomics Studies

9781607616818

9781607616825

b620631

Methods for Rice Phenomics Studies

[Book]

Chyr-Guan Chern Affiliation: Taiwan Agricultural Research Institute, Wufeng, Taichung, Taiwan

With the completion of the rice genome sequencing project, the next major challenge is the large-scale determination of gene function. A systematic phenotypic profiling of mutant collections will provide major insights into gene functions important for crop growth or production. Thus, detailed phenomics analysis is the key to functional genomics. Currently, the two major types of rice mutant collections are insertional mutants and chemical or irradiation-induced mutants. Here we describe how to manipulate a rice mutant population, including conducting phenomics studies and the subsequent propagation and seed storage. We list the phenotypes screened and also describe how to collect data systematically for a database of the qualitative and quantitative phenotypic traits. Thus, data on mutant lines, phenotypes, and segregation rate for all kinds of mutant populations, as well as integration sites for insertional mutant populations, would be searchable, and the collection would be a good resource for rice functional genomics study.

Chyr-Guan Chern Affiliation: Taiwan Agricultural Research Institute, Wufeng, Taichung, Taiwan

Arunee Trisiriroj Affiliation: Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan

Cheng-Chieh Wu Affiliation: Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan

Chyr-Guan Chern Affiliation: Taiwan Agricultural Research Institute, Wufeng, Taichung, Taiwan

Fu-Jin Wei Affiliation: Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan

Ming-Hsing Lai Affiliation: Taiwan Agricultural Research Institute, Wufeng, Taichung, Taiwan

Ming-Jen Fan Affiliation: Department of Biotechnology, Asia University, Taichung, Wufeng, Taiwan

Sheng-Chung Huang Affiliation: Taiwan Agricultural Research Institute, Wufeng, Taichung, Taiwan

Shu Chen Affiliation: Taiwan Agricultural Research Institute, Wufeng, Taichung, Taiwan

Su-May Yu Affiliation: Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan

Yue-Ie C Hsing Affiliation: Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan

[Book]

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