Laboratory techniques in biochemistry and molecular biology.
Cover; Copyright Page ; Preface; TOCContents of parts I and II; Part I: Preparation and Characterisation of Mammalian Plasma Membranes; Contents ; List of abbreviations ; CHChapter 1. Introduction; CHChapter 2. Methods of cell breakage: assessing their suitability and efficacy; CHChapter 3. General methods for the preparation of plasma membranes; CHChapter 4. Identification of subcellular fractions, especially those containing plasma membranes; CHChapter 5. Preparation of plasma membranes from certain tissues and cell lines. Appendix 1. Some properties of equeous sucrose and Ficoll solutions Appendix 2. Rotors ; Appendix 3. Detergents commonly used in membrane analysis ; Appendix 4. Addresses of suppliers ; References ; Subject index; Part II: An Introduction to Affinity Chromatography; Contents ; Preface ; CHChapter 1. An introduction to chromatographic logic. CHChapter 2. Basic concepts in the design of adsorbents for affinity chromatographyCHChapter 3. The chemical technology of affinity chromatography; CHChapter 4. The chromatographic techniques of affinity chromatography; CHChapter 5. Some applications of affinity chromatography in the purification and resolution of proteins and other macromolecules; CHChapter 6. Applications of affinity chromatography to the purification of regulatory macromolecules and complex biological structures; CHChapter 7. Some analytical applications of affinity chromatography. CHChapter 8. Some special techniques of affinity chromatographyAppendix. Manufacturers and suppliers of affinity adsorbents and related materials ; References ; IDXSubject index.