عنوان

Laboratory techniques in biochemistry and molecular biology. / Volume 27, Ultrasensitive and rapid enzyme immunoassay

0080858961

0444502025

1281715859

6611715851

9780080858968

9780444502025

9781281715852

9786611715854

b606630

Laboratory techniques in biochemistry and molecular biology. / Volume 27, Ultrasensitive and rapid enzyme immunoassay

[Book]

Eiji Ishikawa.

Amsterdam ; Oxford

North-Holland Publishing Co. [etc.]

1999

(351 p.).

Laboratory techniques in biochemistry and molecular biology, 27.

Description based upon print version of record.; Chapter 9. Ultrasensitive immunoassay for haptens

Front Cover; Ultrasensitive and Rapid Enzyme Immunoassay; Copyright Page; Contents; Preface; Chapter 1. Introduction: classification of immunoassays; Chapter 2. History of ultrasensitive enzyme immunoassays; Chapter 3. Factors limiting the sensitivity of noncompetitive heterogeneous solid phase enzyme immunoassays; 3.1. Factors limiting the sensitivity to antigens and antibodies; 3.2. Factors limiting the sensitivity to antibodies; 3.3. Factors limiting the sensitivity to antigens; Chapter 4. Methods to minimize effects of factors limiting the sensitivity 4.1. Methods to minimize the nonspecific bindings of antigen- and antibody-enzyme conjugates4.2. Method for efficient trapping of antigens and antibodies; 4.3. Methods using as full reactivities of antigens and antibodies as possible; 4.4. Methods to reduce serum interference; Chapter 5. Principle of the immune complex transfer enzyme immunoassay; 5.1. Immune complex transfer enzyme immunoassay of antibodies; 5.2. Immune complex transfer enzyme inmunoassays of antigens; 5.3 Immune complex transfer enzyme immunoassay for simultaneous detections of antigens and antibodies Chapter 6. Potential of the immune complex transfer enzyme immunoassay to improve the sensitivity and its limitations6.1. Improvement of sensitivity to antibodies; 6.2. Improvement of sensitivity to antigens; 6.3. Simultaneous detection of antigens and antibodies by the immune complex transfer enzyme immunoassay; 6.4. Further improvement of sensitivity; 6.5. Assay variation; 6.6. Factors limiting improvement in sensitivity of the immune complex transfer enzyme immunoassay; Chapter 7. Ultrasensitive and rapid enzyme immunoassay; 7.1 . Shaking for immunoreactions on solid phases 7.2. Use of larger sample volumes7.3. Use of solid phases with larger surfaces; 7.4. Use of circulating thin aqueous layers covering solid phase surfaces; 7.5. Advantages of circulating thin aqueous layer immunoassay; 7.6. Circulating thin aqueous layer immunoassay for antibodies; 7.7. Circulating thin aqueous layer irnmunoassay for antigens; Chapter 8. Protocol of immune complex transfer enzyme immunoassay; 8.1. Choice of enzymes as labels; 8.2. Choice of enzyme-labeling methods; 8.3. Use of inactive forms of enzyme labels 8.4. Preparation of 2,4-dinitrophenyl-bovine serum albumin for immunization8.5. Preparation of biotinyl- and 2,4-dinitrophenyl-bovine serum albumin for irnmunoassays; 8.6. Affinity-purification of (anti-2,4-dinitrophenyl group) IgG; 8.7. Coating of solid phases with proteins; 8.8. Preparations of 2,4-dinitrophenyl.(biotinyl )-antigens and antibodies and antigen- and antibody-enzyme conjugates; 8.9. Typical protocol of immune complex transfer enzyme immunoassay V for antibody IgG; 8.10. Typical protocols of immune complex transfer enzyme immunoassays IX and X for antigens

Immunoassay techniques have become essential in various fields of pure and applied research. This volume of the well known Laboratory Techniques series will be of assistance to those who have plans or are making efforts to develop ultrasensitive enzyme immunoassays for antigens and antibodies. The volume describes factors limiting the sensitivity of noncompetitive solid phase enzyme immunoassays, methods to overcome difficulties limiting sensitivities, methods to perform ultrasensitive enzyme immunoassays as rapidly as possible, and protocols of enzyme labeling and enzyme assays as.

Enzyme-linked immunosorbent assay -- Laboratory manuals.

Molecular biology -- Technique.

Eiji Ishikawa.

Eiji Ishikawa

[Book]

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