عنوان

Plant Molecular Biology Manual

9400909519

9401142173

9789400909519

9789401142175

b592520

Plant Molecular Biology Manual

[Book]

edited by Stanton B. Gelvin, Robbert A. Schilperoort.

Second edition

Dordrecht

Springer Netherlands

2000

(xxi, 161 pages)

E3: Yeast as versatile tool in transcription factor research --; H5: The construction of bacterial artificial chromosome (BAC) libraries --; N1: Gene transfer mediated by site-specific recombination systems --; N2: Use of the R-RS site-specific recombination system in plants --; N3: Cre-lox directed integration of transgenes into the tobacco genome --; 01: Plant actin isolation and characterization.

Plant Molecular Biology Manual (Second Edition) is an entirely new manual containing both fundamental and recently described techniques in the area of plant molecular biology. Designed for use in the research laboratory, the Plant Molecular Biology Manual presents detailed techniques in the areas of plant transformation, recombinant DNA and other nucleic acid manipulations, nuclear run-on and in vitro transcription systems, in situ hybridization and immunodetection systems, protein-nucleic acid interaction analyses, subcellular targeting of proteins in the plant cell, and gene tagging using T-DNA and transposons. This second edition contains more than 40 newly written chapters, including descriptions of subjects such as virus-mediated gene transfer, specialized Agrobacterium strains and T-DNA vectors, nuclear run-on and in vitro transcription systems, non-radioactive detection systems, characterization of transcription factors, nuclear protein targeting, and T-DNA and transposon mutagenesis, not previously described in the first edition.

Botany.

Life sciences.

Plant physiology.

edited by Stanton B. Gelvin, Robbert A. Schilperoort.

Robbert A Schilperoort

Stanton B Gelvin

[Book]

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