a practical guide to scalable recombinant protein production in Escherichia Coli
G. G. Moulton.
Burlington
Elsevier Science
2014
Woodhead Publishing Series in Biomedicine
Description based upon print version of record.
Fed-batch Fermentation is primarily a practical guide for recombinant protein production in E. coli using a Fed-batch Fermentation process. Ideal users of this guide are teaching labs and RetD labs that need a quick and reproducible process for recombinant protein production. It may also be used as a template for the production of recombinant protein product for use in clinical trials. The guide highlights a method whereby a medium cell density - final Ods = 30-40 (A600) - Fed-batch Fermentation process can be accomplished within a single day with minimal supervision. This process can also be d.