عنوان

Basic Exercises in Immunochemistry :

3662001489

9783662001486

b578719

Basic Exercises in Immunochemistry :

[Book]

a Laboratory Manual

by Alois Nowotny.

Berlin, Heidelberg

Springer Berlin Heidelberg

1969

(volumes)

I. Isolation Methods --; Exercise No. 1. Paper Electrophoresis of Human Serum --; Exercise No. 2. Fractionation of Serum Proteins by Ammonium Sulfate --; Exercise No. 3. Fractionation of Serum Proteins by Gel Filtration on Sephadex G 200 --; Exercise No. 4. Column Chromatography of Serum Proteins on DEAE Cellulose --; Exercise No. 5. Dissociation of Macroglobulins with 2-Mercaptoethanol --; Exercise No. 6. Enzymatic Cleavage of Immunoglobulin Fractions --; Exercise No. 7. Chromatographic Separation of the IgG Fragments --; Exercise No. 8. Conjugation of Immune Globulins with Fluorescein and Rhodamine --; Exercise No. 9. Preparation of Soluble Dinitrophenyl (DNP) Proteins --; Exercise No. 10. Isolation of Bacterial H-Antigens --; Exercise No. 11. Extraction of Bacterial O-Antigens (Endotoxins) --; Exercise No. 12. Isolation of Capsular Bacterial Polysaccharides, K Antigens --; Exercise No. 13. Extraction of Teichoic Acid --; Exercise No. 14. Extraction of Pneumococcus Type XIV Polysaccharide --; Exercise No. 15. Isolation of Phytohemagglutinins (Lectins) from Red Kidney Beans. Demonstration of Hemagglutination and Enhancement of Mitosis --; Exercise No. 16. Blood Group Antigen Preparations from Red Blood Cell Stroma --; Exercise No. 17. Isolation of NN Blood Group Antigen from Human Erythrocytes --; Exercise No. 18. Isolation of Forssman Hapten from Erythrocytes --; Exercise No. 19. Ultrasonic Disruption of Bacteria and Density-Gradient Centrifugation of the Products --; Exercise No. 20. Vacuum Distillation and Freeze Drying --; II. Structural Studies --; A. Qualitative Methods --; B. Quantitative Analytical Determinations --; III. Immunological and Other Biological Assays --; A. Antibody Production --; B. Antigen-Antibody Reactions --; Proposed Schedule of Exercises for Teaching Courses in Immunochemistry --; Author Index.

This book intends to be neither a complete survey of the field nor an exhaustive source of references. For these purposes, the use of the extensive compilation "Experimental Immunochemistry" by E.A. KABAT and M.M. MAYER (1962) or the excellent methodological textbook, "Methods in Immunology", by D.H. CAMPBELL, J.S. GARVEY, E.E. CREMER and D.H. SUSSDORF (1963), or the quite comprehensive series "Methods in Immunology and Immunochemistry" by C.A. WILLIAMS and M.W. CHASE (1967) are more suitable. The main purpose of this manual ist to provide students with a simple book which will introduce them to some frequently occurring problems in the three major sections of the immunochemistry of natural products. These are the isolation of the materials, the chemical analysis of the constituents and their structure, and, finally, the assays of the most important biological and immunological activities. In this manual the exercises are simplified and several shortcuts are taken in order to fit them into the framework of a teaching course. The introduction to each exercise gives a brief and elementary explanation of the reaction on which it is based. "Materials and Equipment" lists all tissues or cells, chemicals, glassware, and special equipment which must be available to carry out the exercise, although the very common laboratory tools are usually omitted from the list.

Chemistry.

Life sciences.

Medicine.

by Alois Nowotny.

A Nowotny

[Book]

Y