عنوان

Modern Optics, Electronics and High Precision Techniques in Cell Biology

3642803709

9783642803703

b574114

Modern Optics, Electronics and High Precision Techniques in Cell Biology

[Book]

edited by Gerhard Isenberg.

Berlin, Heidelberg

Springer Berlin Heidelberg

1998

(viii, 261 pages 69 illustrations, 14 illustrations in color.)

Principles and practice.

1 Atomic Force Microscopy Provides Molecular Details of Cell Surfaces --; 2 Basic Principles and Applications of Confocal Laser Scanning Microscopy --; 3 Visualization of Neuronal Form and Function in Brain Slices by Infrared Videomicroscopy --; 4 Time-Resolved Imaging of Membrane Potentials and Cytoplasmic Ions at the Cellular Level with a 50x50 Fiber Array Photodiode Camera --; 5 Micromanipulation of Macromolecules: How to Measure the Stiffness of Single Microtubules --; 6 Dynamics of Single Protein Polymers Visualized by Fluorescence Microscopy --; 7 The Interaction of Proteins with Membrane Surfaces at Molecular Resolution: The Neutron Reflection Method --; 8 The Study of Fast Reactions by the Stopped-Flow Method --; 9 Biomolecular Interactions Analysis (BIA Technology). A Universal Biosensor-Based Technology for Biochemical Research and Development --; 10 Measuring Cellular Locomotion Forces with Micro-Machined Substrates --; 11 Viscoelasticity, Rheology and Molecular Conformational Dynamics of Entangled and Cross-Linked Actin Networks.

The techniques described in this book provide an alternative method to understanding cellular biological structures and processes. Molecular genetic methods mainly analyse single molecules or very small cellular parts, which does not necessarily enhance our understanding of the fundamental cellular processes. However, using techniques such as atomic force or infrared microscopy, neutron reflection, stopped-flow cytometry, laser microscopy, or biosensoric-based electronics, it is possible to study cells and biological systems in their entirety. Physiological processes of cells, such as movement, development, plasticity, regeneration and communication, can be visualized using the high precision biophysical techniques described here.

Biochemistry.

Cytology.

Life sciences.

edited by Gerhard Isenberg.

Gerhard Isenberg

[Book]

Y