عنوان

Medical Biomethods Handbook.

1588292886

9781588292889

b557323

Medical Biomethods Handbook.

[Book]

John M Walker

Totowa, NJ

Humana Press

2005

(656 pages)

Methods in molecular biology.

Preliminary; Basic Techniques in Molecular Biology; Probe Design, Production, and Applications; Restriction EnzymesTools in Clinical Research; Southern Blotting as a Diagnostic Method; Western Blotting as a Diagnostic Method; Principles and Medical Applications of the Polymerase Chain Reaction; Single-Strand Conformation Polymorphism (SSCP) Analysis; Denaturing Gradient Gel Electrophoresis (DGGE); Quantitative Analysis of DNA Sequences by PCR and Solid-Phase Minisequencing; Introduction to Capillary Electrophoresis of DNABiomedical Applications; Mapping Techniques. Dideoxyfingerprinting for Mutation DetectionConformation-Sensitive Gel Electrophoresis; Amplification Refractory Mutation System and Molecular Diagnostics; Ligase Chain Reaction; Chemical Cleavage of MismatchTheory and Clinical Applications; The Protein Truncation Test; Linkage, Allele Sharing, and Association; Single-Nucleotide PolymorphismsTechnology and Applications; cDNA Microarrays; Nucleic Acid Sequence-Based Amplification; Oligonucleotide Ligation Assay; Quantitative TaqMan Real-Time PCRDiagnostic and Scientific Applications. Use of Denaturing High-Performance Liquid Chromatography in Molecular MedicineQuantitative PCR; Liquid Chromatography-Mass Spectrometry of Nucleic Acids; Comparative Genomic Hybridization in Clinical and Medical Research; Bioinformatic Tools for Gene and Protein Sequence Analysis; In SituHybridization; Enzyme-Linked Immunosorbent Assay; Protein TherapeuticsMouse, Humanized, and Human Antibodies; Karyotyping; Microsatellite Analysis; Analysis of Chromosomal Translocations; Differential DisplayTheory and Applications; Techniques for Gene Expression Profiling. Capillary Electrophoresis in Clinical AnalysisFlow Cytometry in the Biomedical Arena; Immunocytochemistry; Ribotyping in Clinical Microbiology; Diagnostic Applications of Protein Microarrays; The Human Genome Project; Prenatal Diagnosis of Inborn Errors of Metabolism; Gene TherapyMethods and Application; Back matter.

John Walker and Ralph Rapley have collected a wide-ranging group of molecular and biochemical techniques that are the most frequently used in medical and clinical research, especially diagnostics. The authors-well-established investigators who run their own research programs and use the methods on a regular basis-outline the practical procedures for using them and describe a variety of pertinent applications. Among the technologies presented are southern and western blotting, electrophoresis, PCR, cDNA and protein microarrays, liquid chromatography, in situ hybridization, karyotyping, flow cyt.

Methods in Molecular Biology

Clinical Laboratory Techniques.

Molecular biology -- Handbooks, manuals, etc.

Molecular diagnosis -- Handbooks, manuals, etc.

John M Walker

John M Walker

Ralph Rapley

[Book]

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